Acetate → HCID 86638818, 화합물

HRID 1620370노트

반응식

HRID 1620370 반응식
[C:1]([O-:4])(=[O:3])[CH3:2].[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10].[P:11]([O-:15])([OH:14])([OH:13])=[O:12].[K+:16]>O>[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[K+:16].[Na+:10].[P:11]([O-:15])([OH:14])([OH:13])=[O:12].[C:1]([O-:4])(=[O:3])[CH3:2].[Na+:10]

참가 물질

5

반응물 3

HCID 175Acetate3회HCID 516951Monobasic potassium phosphate3회HCID 23672064Monosodium phosphate3회

생성물 1

HCID 86638818HCID 86638818, 화합물

시약·촉매·용매 1

HCID 962Water용매

실험 절차

Batch crystallization was performed by admixing around 400 μL of each protein solution with an equal amount of crystallization solution in a 1.5 mL Eppendorff reaction tube. 400 μL of a particular crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution, potassium dihydrogen phosphate stock solution and Milli Q water. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. The following combination of sodium dihydrogen phosphate and potassium dihydrogen phosphate was used: around 0.9 M sodium dihydrogen phosphate and around 0.9 M potassium dihydrogen phosphate. The reaction tubes were stored at ambient temperature. Microscopy of 1 μL aliquots was performed after 11 days.

후처리

  1. customBatch crystallization
  2. customwith an equal amount of crystallization solution in a 1.5 mL
  3. customEppendorff reaction tube
  4. customThe reaction tubes
  5. customwere stored at ambient temperature

출처 및 근거

ORD 기록
ord-57d819e1f65347809c07f9cdc2449ac1
출처 데이터셋
uspto-grants-2014_07
특허 번호
US08772458B2

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