[N:1]([C:8](OC(C)(C)C)=O)([CH3:7])[C@H:2]([C:4](O)=[O:5])[CH3:3].CCN=C=NCCCN(C)C.[CH2:26]([NH:34][C:35]([C@@H:37]1[CH2:41][CH2:40][C@H:39]([CH2:42][CH:43]=[CH2:44])[N:38]1[C:45](=[O:51])[C@@H:46]([NH2:50])[CH2:47][CH:48]=[CH2:49])=[O:36])[CH2:27][C:28]1[CH:33]=[CH:32][CH:31]=[CH:30][CH:29]=1>ClCCl.CN(C1C=CN=CC=1)C>[CH2:26]([NH:34][C:35]([C@@H:37]1[CH2:41][CH2:40][C@H:39]([CH2:42][CH:43]=[CH2:44])[N:38]1[C:45](=[O:51])[C@@H:46]([NH:50][C:4](=[O:5])[C@@H:2]([N:1]([CH3:8])[CH3:7])[CH3:3])[CH2:47][CH:48]=[CH2:49])=[O:36])[CH2:27][C:28]1[CH:29]=[CH:30][CH:31]=[CH:32][CH:33]=1Boc-N-MeAlaOH (5.36 g, 26.4 mmol) is suspended in dichloromethane (200 mL) and EDCI (5.4 g, 28 mmol) and DMAP (3.4 g, 28 mmol) are added to obtain a clear solution. 11G in dichloromethane (50 mL) is added and stirred overnight followed by quenching with saturated bicarb and extracting with dichloromethane then washing with brine, drying over anhydrous magnesium sulfate, filtering and concentrating. LCMS characterization ES+541.2 (m+1).
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