Nhs-LC-biotin → HCID 178508950, 화합물

HRID 1695094

반응식

HRID 1695094 반응식
[NH2:1][C:2]1[N:10]=[C:9]2[C:5]([N:6]=[C:7]([CH2:11][CH2:12][CH2:13][NH2:14])[NH:8]2)=[C:4]([O:15][CH2:16][C:17]2[CH:30]=[CH:29][C:20]([CH2:21][NH:22][C:23](=[O:28])[C:24]([F:27])([F:26])[F:25])=[CH:19][CH:18]=2)[N:3]=1.C1(=O)N([O:36][C:37](=O)[CH2:38][CH2:39][CH2:40][CH2:41][CH2:42][NH:43][C:44](=[O:58])[CH2:45][CH2:46][CH2:47][CH2:48][C@H:49]2[C@@H:57]3[C@@H:52]([NH:53][C:54]([NH:56]3)=[O:55])[CH2:51][S:50]2)C(=O)CC1>CN(C=O)C>[NH2:1][C:2]1[N:10]=[C:9]2[C:5]([N:6]=[C:7]([CH2:11][CH2:12][CH:13]([NH2:14])[C:37](=[O:36])[CH2:38][CH2:39][CH2:40][CH2:41][CH2:42][NH:43][C:44](=[O:58])[CH2:45][CH2:46][CH2:47][CH2:48][C@H:49]3[C@@H:57]4[C@@H:52]([NH:53][C:54]([NH:56]4)=[O:55])[CH2:51][S:50]3)[NH:8]2)=[C:4]([O:15][CH2:16][C:17]2[CH:18]=[CH:19][C:20]([CH2:21][NH:22][C:23](=[O:28])[C:24]([F:26])([F:25])[F:27])=[CH:29][CH:30]=2)[N:3]=1

참가 물질

4

반응물 2

HCID 83874Nhs-LC-biotinHCID 178510944HCID 178510944, 화합물

생성물 1

HCID 178508950HCID 178508950, 화합물수율 86%

시약·촉매·용매 1

HCID 6228Dimethylformamide용매

실험 절차

Amine 41 (Example 24, 10 mg, 0.023 mmol) and N-(+)-biotinyl-6-aminocaproic acid succinimidyl ester (50, 10 mg, 0.023 mmol) are dissolved in 650 μL DMF with 5 μL TEA and left at room temperature over night. The product is purified by reverse phase MPLC by using a linear gradient from water to acetonitrile (0.08% TFA) to yield 15.1 mg (0.019 mmol, 86%) of the title compound. MS (ESI) m/z 763.0 [M+H]+.

후처리

  1. waitleft at room temperature over night
  2. customThe product is purified by reverse phase MPLC

출처 및 근거

ORD 기록
ord-c5fbfebff3d24e53b0add33c7f39919f
출처 데이터셋
uspto-grants-2012_04
특허 번호
US08163479B2

연결된 노트

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