Sodium Carbonate → Biotin

HRID 1748841

반응식

HRID 1748841 반응식
[CH2:1]1[S:5][C@@H:4]([CH2:6][CH2:7][CH2:8][CH2:9][C:10](NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=[O:11])[C@H:3]2[NH:32][C:33]([NH:35][C@@H:2]12)=[O:34].[Na+].C(=O)([O-])[O-:38].[Na+].[Na+]>>[OH:38][C:10]([CH2:9][CH2:8][CH2:7][CH2:6][C@H:4]1[C@@H:3]2[C@@H:2]([NH:35][C:33]([NH:32]2)=[O:34])[CH2:1][S:5]1)=[O:11]

참가 물질

3

반응물 2

HCID 10340Sodium CarbonateHCID 71571497Sulphosuccinimidyl-6-(biotinamido)hexanoate

생성물 1

HCID 171548Biotin

실험 절차

The RA-NAH (10 mg) obtained in the above was dissolved in 0.5 ml of 0.1 M sodium carbonate aqueous solution, subsequently adding 3 mg of sulfo-NHS-LC-biotin (mfd, by Pierce Chemical Company) thereto followed by overnight incubation at 4° C. After overnight dialysis against distilled water thereafter, the dialyzed liquid was lyophilized to obtain the polysaccharide of the present invention in which biotin is bound to the reducing end of NAH (to be referred to as “NAH-biotin” hereinafter) (8 mg).

후처리

  1. customThe RA-NAH (10 mg) obtained in the
  2. customat 4° C
  3. customAfter overnight dialysis
  4. distillationagainst distilled water

출처 및 근거

ORD 기록
ord-1b26caafa6a644a48874c7472e44e884
출처 데이터셋
uspto-grants-2015_10
특허 번호
US09150902B2

연결된 노트

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