Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Fructose 6-phosphate → D-(-)-Fructose
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Fructose 6-phosphate | HCID 69507 | — |
| 반응물 | Erythrose, 4-phosphate | HCID 122357 | — |
| 반응물 | Nadh | HCID 439153 | — |
| 용매 | Tromethamine hydrochloride | HCID 93573 | — |
| 생성물 | D-(-)-Fructose | HCID 5984 | — |
반응 단계
Cell disruption was performed in Tris/HCl buffer (100 mM, pH 7.8). The reaction for determination of transaldolase was performed in 50 mM Tris/HCl buffer (pH 7.8) additionally containing 1 mM fructose 6-phosphate, 0.5 mM NADH, 4 mM erythrose 4-phosphate, 6 U TPI/GDH and 50 μl of crude cell extract. TPI/GDH served as coupling enzymes to allow online monitoring at 340 nm.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US09169502B2 |
| 출처 데이터셋 | uspto-grants-2015_10 |
| ORD ID | ord-05dd12aaed8c491d922b32edbd1a7e07 |
| 출처 메모 | uspto-grants-2015_10 |