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HRID1755210

反应详情

EQUATION

反应方程式

HRID 1755210 的结构方程式

REACTANTS

反应物

3
结构：O=C(CO)[C@@H](O)[C@H](O)[C@H](O)COP(=O)(O)O
HCID69507

Fructose 6-phosphate

C6H13O9P

结构：O=C[C@H](O)[C@H](O)COP(=O)(O)O
HCID122357

D-Erythrose 4-phosphate

C4H9O7P

结构：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
HCID439153

Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide

C21H29N7O14P2

PRODUCTS

生成物

1
结构：O=C(CO)[C@@H](O)[C@H](O)[C@H](O)CO
HCID5984

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
结构：Cl.NC(CO)(CO)CO
溶剂HCID93573

Tromethamine hydrochloride

C4H12ClNO3

2 次

PROCEDURE

实验过程

Cell disruption was performed in Tris/HCl buffer (100 mM, pH 7.8). The reaction for determination of transaldolase was performed in 50 mM Tris/HCl buffer (pH 7.8) additionally containing 1 mM fructose 6-phosphate, 0.5 mM NADH, 4 mM erythrose 4-phosphate, 6 U TPI/GDH and 50 μl of crude cell extract. TPI/GDH served as coupling enzymes to allow online monitoring at 340 nm.

WORKUP

后处理

  1. customThe reaction for determination of transaldolase
  2. extractionextract