Alpha-Ketoglutarate → Oxalacetate

HRID 1769591

반응식

HRID 1769591 반응식
[NH2:1][C@H:2]([C:7]([O-:9])=[O:8])[CH2:3][C:4]([O-:6])=[O:5].[O:10]=[C:11]([CH2:15][CH2:16][C:17]([O-:19])=[O:18])[C:12]([O-:14])=[O:13]>>[C:4]([O-:6])(=[O:5])[CH2:3][C:2]([C:7]([O-:9])=[O:8])=[O:10].[NH2:1][C@H:11]([C:12]([O-:14])=[O:13])[CH2:15][CH2:16][C:17]([O-:19])=[O:18]

참가 물질

4

반응물 2

HCID 164533Alpha-KetoglutarateHCID 5460541l-aspartic acid, sodium salt

생성물 2

HCID 164550OxalacetateHCID 5460544L-glutamate(2-)

실험 절차

PRINCIPLE ##STR5## AST catalyzes the transfer of the amino group from aspartate to 2-oxoglutarate to yield oxalacetate and glutamate. The oxalacetate is reduced to malate in the presence of malate dehydrogenase [MD] with the simultaneous oxidation of reduced nicotinamide adenine dinucleotide [NADH]. The rate of decrease in absorbance at 340 nm is directly proportional to AST activity.

출처 및 근거

ORD 기록
ord-01a7a9d20ed64ae891a8cf0d3bd44d71
출처 데이터셋
uspto-grants-1995_01
특허 번호
US05382525

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