Diisopropylethylamine → 5-(3-((1-(1,2,4-oxadiazol-3-yl)cyclobutyl)carbamoyl)phenyl)-2-(4-fluorophenyl)-N-methyl-6-((2,2,2-trifluoroethyl)amino)furo[2,3-b]pyridine-3-carboxamide
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CN([C:4]([O:8][N:9]1N=[N:16][C:11]2[CH:12]=[CH:13][CH:14]=[N:15][C:10]1=2)=[N+](C)C)C.F[P-](F)(F)(F)(F)F.[F:25][C:26]1[CH:31]=[CH:30][C:29]([C:32]2[O:55][C:35]3=[N:36][C:37]([NH:49][CH2:50][C:51]([F:54])([F:53])[F:52])=[C:38]([C:40]4[CH:41]=[C:42]([CH:46]=[CH:47][CH:48]=4)[C:43]([OH:45])=O)[CH:39]=[C:34]3[C:33]=2[C:56](=[O:59])[NH:57][CH3:58])=[CH:28][CH:27]=1.C(N(C(C)C)C(C)C)C.Cl.O1C=NC(C2(N)CCC2)=N1>CN(C=O)C>[O:8]1[CH:4]=[N:15][C:10]([C:11]2([NH:16][C:43]([C:42]3[CH:41]=[C:40]([C:38]4[CH:39]=[C:34]5[C:33]([C:56]([NH:57][CH3:58])=[O:59])=[C:32]([C:29]6[CH:28]=[CH:27][C:26]([F:25])=[CH:31][CH:30]=6)[O:55][C:35]5=[N:36][C:37]=4[NH:49][CH2:50][C:51]([F:54])([F:53])[F:52])[CH:48]=[CH:47][CH:46]=3)=[O:45])[CH2:12][CH2:13][CH2:14]2)=[N:9]1참가 물질
반응물 4
HCID 81531Diisopropylethylamine
HCID 9886157Methanaminium, N-((dimethylamino)(3H-1,2,3-triazolo(4,5-b)pyridin-3-yloxy)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1)
HCID 904023871-(1,2,4-Oxadiazol-3-yl)cyclobutan-1-amine hydrochloride
HCID 904025453-(2-(4-Fluorophenyl)-3-(methylcarbamoyl)-6-((2,2,2-trifluoroethyl)amino)furo[2,3-b]pyridin-5-yl)benzoic acid생성물 1
시약·촉매·용매 1
실험 절차
2-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)-1,1,3,3-tetramethylisouronium hexafluorophosphate(V) (29 mg, 0.077 mmol) was added to stirring solution of 3-(2-(4-fluorophenyl)-3-(methylcarbamoyl)-6-((2,2,2-trifluoroethyl)amino)furo[2,3-b]pyridin-5-yl)benzoic acid (25 mg, 0.051 mmol), N-ethyl-N-isopropylpropan-2-amine (0.027 mL, 0.15 mmol) and 1-(1,2,4-oxadiazol-3-yl)cyclobutanamine hydrochloride (14 mg, 0.077 mmol) in DMF (1 mL) at rt. The mixture was allowed to stir at rt. for 30 min. The crude material was purified via preparative LC/MS with the following conditions: Column:Waters XBridge C18, 19×200 mm, 5-μm particles; Mobile Phase A: water with 20-mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile:water with 20-mM ammonium acetate; Gradient: 40-80% B over 20 minutes, then a 5-minute hold at 100% B; Flow: 20 mL/min. Fractions containing the desired product were combined and dried via centrifugal evaporation. The yield of the product was 4.0 mg, and its estimated purity by LCMS analysis was 100%. Two analytical LC/MS injections were used to determine the final purity. Injection 1 conditions: Column:Waters Acquity UPLC BEH C18, 2.1×50 mm, 1.7-μm particles; Mobile Phase A: 5:95 acetonitrile:water with 10 mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile:water with 10 mM ammonium acetate; Temperature: 50° C.; Gradient: 0-100% B over 3 minutes, then a 0.75-minute hold at 100% B; Flow: 1.11 mL/min. retention time: 2.95 min; M+H: 609. Injection 2 conditions: Column:Waters Acquity UPLC BEH C18, 2.1×50 mm, 1.7-μm particles; Mobile Phase A: 5:95 acetonitrile:water with 0.05% TFA; Mobile Phase B: 95:5 acetonitrile:water with 0.1% TFA; Temperature: 50° C.; Gradient: 0-100% B over 3 minutes, then a 0.75-minute hold at 100% B; Flow: 1.11 mL/min. retention time: 3.02 min; M+H: 609. Proton NMR was acquired in deuterated DMSO. 1H NMR (500 MHz, DMSO-d6) δ 9.49 (s, 1H), 9.31 (s, 1H), 8.42-8.32 (m, 1H), 8.01-7.90 (m, 4H), 7.69 (s, 1H), 7.66-7.55 (m, 2H), 7.39-7.29 (m, 2H), 6.65-6.59 (m, 1H), 4.23-4.11 (m, 2H), 2.79 (d, J=4.3 Hz, 3H), 2.68-2.55 (m, 4H), 2.10-2.00 (m, 2H).
후처리
- customThe crude material was purified via preparative LC/MS with the following conditions
- waitGradient: 40-80% B over 20 minutes
- customa 5-minute hold
- additionFractions containing the desired product
- customdried via centrifugal evaporation
- custom50° C.
- waitGradient: 0-100% B over 3 minutes
- customa 0.75-minute hold
- custom50° C.
- waitGradient: 0-100% B over 3 minutes
- customa 0.75-minute hold
출처 및 근거
- ORD 기록
- ord-1f33966bf71949f7bc1d8e5f1c8d8bfc
- 출처 데이터셋
- uspto-grants-2015_02
- 특허 번호
- US08962651B2
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