[OH-].[Na+].[CH:3]([N:6]1[C:10]2[N:11]=[C:12]([N:20]3[CH2:25][CH2:24][N:23]([CH3:26])[CH2:22][CH2:21]3)[CH:13]=[C:14]([C:15]([O:17]CC)=[O:16])[C:9]=2[CH:8]=[N:7]1)([CH3:5])[CH3:4]>CCO>[CH:3]([N:6]1[C:10]2[N:11]=[C:12]([N:20]3[CH2:25][CH2:24][N:23]([CH3:26])[CH2:22][CH2:21]3)[CH:13]=[C:14]([C:15]([OH:17])=[O:16])[C:9]=2[CH:8]=[N:7]1)([CH3:5])[CH3:4]Aqueous NaOH (0.193 g, 4.833 mmol) was added to a solution of ethyl 1-isopropyl-6-(4-methylpiperazin-1-yl)-1H-pyrazolo[3,4-b]pyridine-4-carboxylate (0.32 g, 0.966 mmol) in EtOH (6 mL) and stirred at 60° C. for 1 h. After completion of the reaction, ethanol was removed under reduced pressure and it was acidified using 10% citric acid solution. Extraction was carried out using DCM; the combined organic layers were washed with water, brine; dried over anhydrous Na2SO4; filtered and concentrated under reduced pressure to give the desired compound (0.2 g, 64.9%).
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