Platinum → HCID 178443123, 화합물

HRID 1954439

반응식

HRID 1954439 반응식
[Pt:1].[C:2]([O:19]C(=O)CCCCCCCCCCCCCCC)(=O)[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]>C(=O)(O)[O-].C([NH+](CC)CC)C.C(O)(C)C.O.[Pd]>[C:2]([Pt:1])(=[O:19])[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH3:17]

참가 물질

6

반응물 2

HCID 23939PlatinumHCID 69339Palmitic anhydride

생성물 1

HCID 178443123HCID 178443123, 화합물

시약·촉매·용매 3

HCID 23938Palladium촉매HCID 11768884Triethylammonium hydrogen carbonate용매HCID 21889133Propan-2-ol--water (1/1)용매

실험 절차

Preparation used the same procedure as the platinum cluster conjugate: an estimated 30 nmol of amino-derivatized 2-3 nm palladium cluster, dissolved in 0.6 M triethylammonium bicarbonate in 20% isopropanol/water, was evaporated to dryness five times from methanol, dissolved in 3 mL of dichloromethane, then treated with 200 nmol of palmitic anhydride. The mixture was stirred for one hour at room temperature, washed three times with 0.1 M sodium phosphate buffer at pH 6.5, evaporated to dryness, redissolved in 2:1 methanol/chloroform and separated by gel filtration (GH25, Amicon, eluted with 2:1 methanol:chloroform). After evaporation to dryness, lipid conjugation was demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate was shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent.

후처리

  1. customPreparation
  2. customwas evaporated to dryness five times from methanol
  3. dissolutiondissolved in 3 mL of dichloromethane
  4. washwashed three times with 0.1 M sodium phosphate buffer at pH 6.5
  5. customevaporated to dryness
  6. dissolutionredissolved in 2:1 methanol/chloroform
  7. customseparated by gel filtration (GH25, Amicon
  8. washeluted with 2:1 methanol
  9. customAfter evaporation to dryness, lipid conjugation
  10. extractionwas demonstrated by extraction of the cluster label into the chloroform layer when the palmitoyl palladium cluster conjugate
  11. stirringwas shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent

출처 및 근거

ORD 기록
ord-cd88928292c946de8958728c9d98dd56
출처 데이터셋
uspto-grants-2000_09
특허 번호
US06121425

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