L-Alanine → L-Phenylalanine

HRID 1957

반응식

N[C@H](C(O)=O)C.[NH2:7][C@H:8]([C:16]([OH:18])=[O:17])[CH2:9][C:10]1[CH:15]=[CH:14][CH:13]=[CH:12][CH:11]=1.N[C@@H](C(O)=O)C.C1(CC(=O)C([O-])=O)C=CC=CC=1>>[NH2:7][C@@H:8]([C:16]([OH:18])=[O:17])[CH2:9][C:10]1[CH:15]=[CH:14][CH:13]=[CH:12][CH:11]=1.[NH2:7][C@H:8]([C:16]([OH:18])=[O:17])[CH2:9][C:10]1[CH:15]=[CH:14][CH:13]=[CH:12][CH:11]=1

참가 물질

반응물 4

HCID 5950L-Alanine

HCID 6140L-Phenylalanine

HCID 71080D-Alanine

HCID 45926973-Phenylpyruvate

생성물 2

HCID 6140L-Phenylalanine

HCID 71567D-Phenylalanine

실험 절차

Using cultures of recombinant cells of the present invention with the addition of D-, L-alanine and L-phenylalanine as additional sources of D-alanine and phenylpyruvate substrates for the D-aminotransferase gene product resulted in the production of 13.66 g/l of D-phenylalanine and 0.47 g/l of L-phenylalanine, a 94% enantiomeric excess. In the case where only D-, L-alanine was added to the cultures during the fermentation process resulted in the production of 4.15 g/l of D-phenylalanine and no L-phenylalanine, a 100% enantiomeric excess. In contrast, when no D-, L-alanine or L-phenylalanine was added to the cell cultures during the fermentation process, 1.12 g/l of D-phenylalanine and 0.47 g/l of L-phenylalanine is produced, a 41% enantiomeric excess.

출처 및 근거

ORD 기록: ord-5b62ce49ffab4c1db9e358eac8f40d5a
출처 데이터셋: uspto-grants-1998_03
DOI: 10.6084/m9.figshare.5104873.v1
특허 번호: US05728555

연결된 노트

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