Camp → Adenosine Monophosphate
반응식
[CH:1]1[N:2]=[C:3]([NH2:22])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@@H:13]4[CH2:15][O:16][P:17]([OH:20])([O:19][C@H:12]4[C@H:11]3[OH:21])=[O:18])[C:5]=2[N:6]=1.[CH:23]1[N:27]([C@@H:28]2[O:32][C@@H:31]3[CH2:33][O:34][P:35]([OH:38])([O:37][C@H:30]3[C@H:29]2[OH:39])=[O:36])[C:26]2[NH:40][C:41]([NH2:45])=[N:42][C:43](=[O:44])[C:25]=2[N:24]=1>>[CH:1]1[N:2]=[C:3]([NH2:22])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([OH:20])([OH:19])=[O:18])[C@@H:12]([OH:32])[C@H:11]3[OH:21])[C:5]=2[N:6]=1.[CH:23]1[N:27]([C@@H:28]2[O:32][C@H:31]([CH2:33][O:34][P:35]([OH:38])([OH:37])=[O:36])[C@@H:30]([OH:14])[C@H:29]2[OH:39])[C:26]2[NH:40][C:41]([NH2:45])=[N:42][C:43](=[O:44])[C:25]=2[N:24]=1참가 물질
4
실험 절차
The enzyme of the present invention isolated and purified from rat cerebral cortex in Example was acted on [8-3H] cAMP and [8-3H] cGMP as substrates to form [8-3H] 5'-AMP and [8-3H] 5'-GMP, respectively. The products were reacted with 5'-nucleotidase to form [8-3H] adenosine and [8-3H] guanosine, respectively. These products were separated from each other on a cation exchange resin column (AG50 W-X4, Bio-Rad) to assay for the radioactivity. The assay was carried out in a manner similar to that of the enzymatic activity described in Example.