Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Sucrose → Spectinomycin
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Sucrose | HCID 5988 | — |
| 반응물 | 1-Naphthaleneacetic Acid | HCID 6862 | — |
| 반응물 | Spectinomycin Dihydrochloride | HCID 64740 | — |
| 반응물 | N-Benzyl-7H-purin-2-amine | HCID 12922494 | — |
| 생성물 | Spectinomycin | HCID 15541 | — |
반응 단계
When calli are about 0.5 mm in size they are transferred to callus growth medium SH2 (Schenk & Hildebrandt, Can. J. Bot. 50:199-204 (1972)) with 30 g/l saccharose, 5 g/l agarose, 0.1 mg/l 1-naphtalene acetic acid (NAA) and 0.1 mg/l benzylaminopurin (BAP), and the selecting agent spectinomycin dihydrochloride at concentrations of 0-1000 mg/l. It was found that the optimal concentration of selection is 500 mg/l. The non-resistant calli appear as white calli. They also grow slower as compared to the control calli. The culture conditions are as described above for the above protoplast calli.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US07645918B2 |
| 출처 데이터셋 | uspto-grants-2010_01 |
| ORD ID | ord-4533c4ee90ef4d7297740a8da5a3b712 |
| 출처 메모 | uspto-grants-2010_01 |