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HRID1991591

反应详情

EQUATION

反应方程式

HRID 1991591 的结构方程式

REACTANTS

反应物

4
结构：OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O
HCID5988

Sucrose

C12H22O11

结构：O=C(O)Cc1cccc2ccccc12
HCID6862

1-Naphthaleneacetic acid

C12H10O2

结构：CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O.Cl.Cl
HCID64740

Spectinomycin dihydrochloride

C14H26Cl2N2O7

结构：c1ccc(CNc2ncc3[nH]cnc3n2)cc1
HCID12922494

N-Benzyl-7H-purin-2-amine

C12H11N5

PRODUCTS

生成物

1
结构：CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O
HCID15541

Spectinomycin

C14H24N2O7

PROCEDURE

实验过程

When calli are about 0.5 mm in size they are transferred to callus growth medium SH2 (Schenk & Hildebrandt, Can. J. Bot. 50:199-204 (1972)) with 30 g/l saccharose, 5 g/l agarose, 0.1 mg/l 1-naphtalene acetic acid (NAA) and 0.1 mg/l benzylaminopurin (BAP), and the selecting agent spectinomycin dihydrochloride at concentrations of 0-1000 mg/l. It was found that the optimal concentration of selection is 500 mg/l. The non-resistant calli appear as white calli. They also grow slower as compared to the control calli. The culture conditions are as described above for the above protoplast calli.

WORKUP

后处理

  1. concentrationIt was found that the optimal concentration of selection