N-Acetyl-D-Glucosamine → D-Glucosamine

반응식

Cl.N1C2C(=CC=CC=2)C=C1.C([NH:14][C@@H:15]1[C@@H:21]([OH:22])[C@H:20]([OH:23])[C@@H:19]([CH2:24][OH:25])[O:18][CH:16]1[OH:17])(=O)C>>[OH:17][CH:16]1[O:18][C@H:19]([CH2:24][OH:25])[C@@H:20]([OH:23])[C@H:21]([OH:22])[C@H:15]1[NH2:14]

참가 물질

반응물 2

HCID 439174N-Acetyl-D-Glucosamine

HCID 57447477Indole hydrochloride

생성물 1

HCID 439213D-Glucosamine

실험 절차

An enzyme solution (0.1 ml) is added to 0.3 ml of 100 mM phosphate buffer (pH 7.8) containing 0.1 ml of 1% N-acetyl-D-glucosamine as a substrate, and the resultant mixture is incubated at 37° C. for 30 minutes. The amount of D-glucosamine formed is determined colorimetrically using indole-HCl according to the method of Z. Dische and E. Borenfreund [J. Biol. Chem., 184:517 (1950)]. One unit of the enzyme is defined as the amount of the enzyme which is capable of producing 1 μmol D-glucosamine from N-acetyl-D-glucosamine per minute at 37° C.

출처 및 근거

ORD 기록: ord-78ad018610204225840c56fed8dc6e9d
출처 데이터셋: uspto-grants-1995_06
DOI: 10.6084/m9.figshare.5104873.v1
특허 번호: USH0001453

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