Crystal Violet → Neutral Red Crystal Violet

HRID 218291

반응식

HRID 218291 반응식
C[C@@H](O)[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](N)[C@@H](O)[C@@H]1O.[CH3:35][C:36]1[C:52]([NH2:53])=[CH:51][C:50]2[C:38](=[N:39][C:40]3[CH:45]=[CH:44][C:43]([N:46]([CH3:48])[CH3:47])=[CH:42][C:41]=3[N:49]=2)[CH:37]=1.[ClH:54].[CH3:55][N:56]([C:58]1[CH:63]=[CH:62][C:61]([C:64]([C:74]2[CH:79]=[CH:78][C:77]([N:80]([CH3:82])[CH3:81])=[CH:76][CH:75]=2)=[C:65]2[CH:73]=[CH:72][C:68](=[N+:69]([CH3:71])[CH3:70])[CH:67]=[CH:66]2)=[CH:60][CH:59]=1)[CH3:57].[Cl-]>>[CH3:35][C:36]1[C:52]([NH2:53])=[CH:51][C:50]2[C:38](=[N:39][C:40]3[CH:45]=[CH:44][C:43]([N:46]([CH3:48])[CH3:47])=[CH:42][C:41]=3[N:49]=2)[CH:37]=1.[ClH:54].[CH3:71][N:69]([C:68]1[CH:67]=[CH:66][C:65]([C:64]([C:61]2[CH:60]=[CH:59][C:58]([N:56]([CH3:55])[CH3:57])=[CH:63][CH:62]=2)=[C:74]2[CH:75]=[CH:76][C:77](=[N+:80]([CH3:82])[CH3:81])[CH:78]=[CH:79]2)=[CH:73][CH:72]=1)[CH3:70].[Cl-:54]

참가 물질

4

반응물 3

HCID 11057Crystal VioletHCID 11105Neutral RedHCID 123865Geneticin

생성물 1

HCID 131729771Neutral Red Crystal Violet

실험 절차

The survival of transfected cells in the absence or presence of G418 was monitored by staining of foci/clones with neutral red in vivo with subsequent crystal violet staining. The medium was removed from the cells and replaced with fresh medium containing 0.0025% neutral red (Sigma N2889) and the cells incubated for 3 hours at 37° C. Cells were washed twice with PBS, fixed in 3.5% formaldehyde for 15 minutes, washed twice again in PBS and then with distilled water and the number of (live) foci counted. The cells could then be re-stained with crystal violet by incubating with an 0.1% crystal violet (Sigma C0775) solution in 20% methanol for 20 minutes at room temperature, followed by 3 washes in 20% methanol and a wash with distilled water.

후처리

  1. customThe medium was removed from the cells
  2. additioncontaining 0.0025% neutral red (Sigma N2889)
  3. washCells were washed twice with PBS
  4. waitfixed in 3.5% formaldehyde for 15 minutes
  5. washwashed twice again in PBS
  6. waitby incubating with an 0.1% crystal violet (Sigma C0775) solution in 20% methanol for 20 minutes at room temperature
  7. washin 20% methanol and a wash with distilled water

출처 및 근거

ORD 기록
ord-9895c8a70eb04a8e8d995d781a84ed98
출처 데이터셋
uspto-grants-2012_06
특허 번호
US08198426B2

연결된 노트

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