반응식
[CH2:1]1[CH2:8][C@@H:7]([NH2:9])[C:5](=[O:6])[NH:4][CH2:3][CH2:2]1.CC1C(O)=C(C=O)C(COP(O)(O)=O)=CN=1.OP(O)(O)=O>>[CH2:1]1[CH2:8][C@H:7]([NH2:9])[C:5](=[O:6])[NH:4][CH2:3][CH2:2]1.[CH2:1]1[CH2:8][C@@H:7]([NH2:9])[C:5](=[O:6])[NH:4][CH2:3][CH2:2]1참가 물질
5
실험 절차
Then D-α-amino-ε-caprolactam and pyridoxal-5-phosphate were added to the disintegrated cell suspensions of each monoculture to concentrations of 5 g/l and 0.01 mM respectively. After 1.5 h of incubation at 20° C. and 20 rpm, 2 ml samples were transferred into 1 ml of 1 M H3PO4. After removal of all particulates by centrifugation followed by filtration through a 0.22 μM filter, concentrations D- and L-α-amino-ε-caprolactam in the samples were determined by HPLC using the protocol as described in Example 1. Finally, monocultures that formed L-α-amino-ε-caprolactam from the D-α-amino-ε-caprolactam substrate were sent to the NCIMB for strain determination.
후처리
- customAfter removal of all particulates by centrifugation
- filtrationfollowed by filtration through a 0.22 μM
- filtrationfilter
- concentrationconcentrations D- and L-α-amino-ε-caprolactam in the samples
출처 및 근거
- ORD 기록
- ord-588393c3f68845bbb4df505aac40a8bc
- 출처 데이터셋
- uspto-grants-2008_05
- 특허 번호
- US07371555B2
연결된 노트
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