Epinephrine → (2S)-3-(4-hydroxyphenyl)-2-nitramidopropanoic acid

HRID 2414230

반응식

HRID 2414230 반응식
CCCCC[C@H](O)/C=C/[C@@H]1[C@@H](C/C=C\CCC[C:20]([OH:22])=[O:21])[C@H]2OO[C@@H]1C2.C(O)(=[O:46])CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC.C[NH:49][CH2:50][C@H:51](O)[C:52]1[CH:53]=[CH:54][C:55]([OH:59])=[C:56](O)[CH:57]=1.C(N)CN([N:67]([OH:70])N=O)CCN>P([O-])([O-])([O-])=O.CC1C2[N-]C(=CC3[N-]C(C=C4N=C(C=C5N=C(C=2)C(C=C)=C5C)C(C=C)=C4C)=C(C)C=3CCC(O)=O)C=1CCC(O)=O.O.[Fe+2]>[N+:67]([NH:49][C@H:50]([C:20]([OH:22])=[O:21])[CH2:51][C:52]1[CH:53]=[CH:54][C:55]([OH:59])=[CH:56][CH:57]=1)([O-:70])=[O:46]

참가 물질

7

반응물 4

HCID 5816EpinephrineHCID 444899Arachidonic AcidHCID 445049Prostaglandin H2HCID 178511350HCID 178511350, 화합물

생성물 1

HCID 9834478(2S)-3-(4-hydroxyphenyl)-2-nitramidopropanoic acid

시약·촉매·용매 2

HCID 27337Ferriheme hydroxide촉매HCID 1061Phosphate용매

실험 절차

Reaction mixture in 100 mM phosphate buffer, pH 7.4, (total volume, 200 μl) was incubated at 37° C. for 30 minutes. The reaction mixture contained a combination of cytochrome c (40 μg), ovine prostaglandin H2 synthase form 2 (104 units), arachidonic acid (590 μM), epinephrine (31 μg), hematin (1 μM), and DETA NONOate, an NO donor, (300 μM) as indicated in FIG. 2. After incubation, the proteins were separated by SDS-PAGE followed by Western blot analysis with an alkaline phosphatase system using monoclonal antibodies produced against nitrotyrosine.

후처리

  1. customAfter incubation, the proteins were separated by SDS-PAGE

출처 및 근거

ORD 기록
ord-76199e79b92a4202aea748587ed7142b
출처 데이터셋
uspto-grants-2004_11
특허 번호
US06812212B2

연결된 노트

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