Trifluoroacetic acid → Lys-Val-Lys

HRID 251316

반응식

HRID 251316 반응식
CC(OC([NH:8][CH2:9][CH2:10][CH2:11][CH2:12][C@H:13]([NH2:17])[C:14]([OH:16])=[O:15])=O)(C)C.[NH:18]([C:26]([O:28]CC1C2C(=CC=CC=2)C2C1=CC=CC=2)=O)[C@H:19]([C:23]([OH:25])=O)[CH:20]([CH3:22])[CH3:21].[NH:43](C(OCC1C2C(=CC=CC=2)C2C1=CC=CC=2)=O)[C@H:44](C(O)=O)[CH2:45][CH2:46][CH2:47][CH2:48][NH:49]C(OC(C)(C)C)=O.C(O)(C(F)(F)F)=O>CCOCC>[NH2:43][C@H:44]([C:26]([NH:18][C@H:19]([C:23]([NH:17][C@H:13]([C:14]([OH:16])=[O:15])[CH2:12][CH2:11][CH2:10][CH2:9][NH2:8])=[O:25])[CH:20]([CH3:21])[CH3:22])=[O:28])[CH2:45][CH2:46][CH2:47][CH2:48][NH2:49]

참가 물질

6

반응물 4

HCID 6422Trifluoroacetic acidHCID 688217FMOC-L-valineHCID 2724628Fmoc-Lys(Boc)-OHHCID 2733283(2S)-2-amino-6-(((tert-butoxy)carbonyl)amino)hexanoic acid

생성물 1

HCID 448140Lys-Val-Lys

시약·촉매·용매 1

HCID 3283Diethyl Ether용매

실험 절차

The protective peptide is built on 1.00 g (0.80 mmol) of H-Lys(Boc)-2-chlorotrityl resin using Fmoc-Val-OH, Fmoc-Lys(Boc)-OH and Palm-OSu. The resin is treated for 30 min with 8 ml of TFA 95% and the solution is instilled in 100 ml of Et2O. The precipitate is sucked off, washed and purified by prep. HPLC after drying. The substance is diluted in 30 ml of dioxan:water=4:6, treated overnight with 2.0 g of BioRad resin (acetate form), filtered, rotated and lyophilized. Yield: 110 mg (0.15 mmol, 19%)

후처리

  1. washwashed
  2. custompurified by prep
  3. customHPLC after drying
  4. additionThe substance is diluted in 30 ml of dioxan
  5. additionwater=4:6, treated overnight with 2.0 g of BioRad resin (acetate form)
  6. filtrationfiltered

출처 및 근거

ORD 기록
ord-73c73a56b2d444b7857252e72f7b484d
출처 데이터셋
uspto-grants-2011_01
특허 번호
US07863417B2

연결된 노트

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