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[CH:1]1[CH:6]=[N+:5]([C@@H:7]2[O:11][C@H:10]([CH2:12][O:13][P:14]([O:17][P:18]([O:21][CH2:22][C@H:23]3[O:27][C@@H:26]([N:28]4[C:32]5[N:33]=[CH:34][N:35]=[C:36]([NH2:37])[C:31]=5[N:30]=[CH:29]4)[C@H:25]([O:38][P:39]([OH:42])([OH:41])=[O:40])[C@@H:24]3[OH:43])([OH:20])=[O:19])([OH:16])=[O:15])[C@@H:9]([OH:44])[C@H:8]2[OH:45])[CH:4]=[C:3]([C:46]([NH2:48])=[O:47])[CH:2]=1.O=C[C@H]([C@@H]([C@@H]([C@H](C)O)O)O)O>>[CH:34]1[N:35]=[C:36]([NH2:37])[C:31]2[N:30]=[CH:29][N:28]([C@@H:26]3[O:27][C@H:23]([CH2:22][O:21][P:18]([O:17][P:14]([O:13][CH2:12][C@H:10]4[O:11][C@@H:7]([N:5]5[CH:4]=[C:3]([C:46]([NH2:48])=[O:47])[CH2:2][CH:1]=[CH:6]5)[C@H:8]([OH:45])[C@@H:9]4[OH:44])([OH:16])=[O:15])([OH:20])=[O:19])[C@@H:24]([OH:43])[C@H:25]3[O:38][P:39]([OH:42])([OH:41])=[O:40])[C:32]=2[N:33]=1참가 물질
3
실험 절차
To 2.5 ml of Tris-imidazole-sodium acetate buffer solution (solution containing 0.12 M each is adjusted to pH of 9.5 with 4 N NaOH) is added 0.2 ml of 15 mM NADP+ solution After keeping at 37° C. for 5 minutes, 0.1 ml of an enzyme solution is added and 0.2 ml of 150 mM L-fucose solution is further added to the system to initiate the reaction. Immediately thereafter, the reaction mixture is transferred to a cell for measurement of absorbance (1 cm light path) kept at 37° C. and absorbance is measured at the wavelength of 340 nm for 2 minutes or, if necessary, over a longer time period. One unit is an amount of enzyme that produces 1 μmole of NADPH for one minute.
후처리
- customthe reaction
- customkept at 37° C.
- waitabsorbance is measured at the wavelength of 340 nm for 2 minutes
출처 및 근거
- ORD 기록
- ord-675af41d9154400694a56d97e187d2ff
- 출처 데이터셋
- uspto-grants-1993_11
- 특허 번호
- US05260197
연결된 노트
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