D-Alanine → DOTA biotin

HRID 277617

반응식

HRID 277617 반응식
N[C@@H:2]([C:4]([OH:6])=[O:5])[CH3:3].C(N[C@@H](C(O)=O)C)(=O)CC[CH2:10][CH2:11][C@H:12]1[C@@H:20]2[C@@H:15]([NH:16][C:17]([NH:19]2)=[O:18])[CH2:14][S:13]1.Cl.CN(C)CCCN=C=NCC.ON1C(=O)CCC1=O.[CH2:48]1[N:59]([CH2:60][C:61]([OH:63])=[O:62])[CH2:58][CH2:57][N:56]([CH2:64][C:65]([OH:67])=[O:66])[CH2:55][CH2:54][N:53]([CH2:68][C:69]([OH:71])=[O:70])[CH2:52][CH2:51][N:50]([CH2:72][C:73]([OH:75])=[O:74])[CH2:49]1.NC1C=CC=CC=1>>[CH2:49]1[N:50]([CH2:72][C:73]([OH:75])=[O:74])[CH2:51][CH2:52][N:53]([CH2:68][C:69]([OH:71])=[O:70])[CH2:54][CH2:55][N:56]([CH2:64][C:65]([OH:67])=[O:66])[CH2:57][CH2:58][N:59]([CH2:60][C:61]([OH:63])=[O:62])[CH2:48]1.[OH:6][C:4]([CH2:2][CH2:3][CH2:10][CH2:11][C@H:12]1[C@@H:20]2[C@@H:15]([NH:16][C:17]([NH:19]2)=[O:18])[CH2:14][S:13]1)=[O:5]

참가 물질

6

반응물 5

HCID 71080D-Alanine2회HCID 80170N-Hydroxysuccinimide2회HCID 27239391,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochlorideHCID 22863182Biotinyl-d-alanineHCID 131719895DOTA aniline

생성물 1

HCID 87364501DOTA biotin

실험 절차

The D-alanine-linked conjugate was prepared by first coupling D-alanine (Sigma Chemical Co.) to biotin-NHS ester. The resultant biotinyl-D-alanine was then activated with 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDCI) and N-hydroxysuccinimide (NHS). This NHS ester was reacted in situ with DOTA-aniline to give the desired product which was purified by preparative HPLC.

출처 및 근거

ORD 기록
ord-de74687018da4a6989aa8c9929cb9295
출처 데이터셋
uspto-grants-2002_03
특허 번호
US06358490B2

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