Biotin N-hydroxysuccinimide ester → Biotin

HRID 344635노트

반응식

HRID 344635 반응식
C1C(=O)N([O:8][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@@H:15]2[S:19][CH2:18][C@@H:17]3[NH:20][C:21]([NH:23][C@H:16]23)=[O:22])=[O:10])C(=O)C1>CN(C=O)C.C([O-])(O)=O.[Na+]>[OH:10][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@H:15]1[C@@H:16]2[C@@H:17]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:19]1)=[O:8]

참가 물질

4

반응물 1

HCID 6710714Biotin N-hydroxysuccinimide ester4회

생성물 1

HCID 171548Biotin

시약·촉매·용매 2

HCID 6228Dimethylformamide용매HCID 516892Sodium Bicarbonate용매

실험 절차

Histone H1 was biotinylated to different degrees with increasing amounts of BHSE. 5 μg BHSE (dissolved in 10 μl DMF), 25 μg BHSE (in 10 μl DMF) and 250 μg BHSE in 10μl DMF were added to three solutions containing the same amount of histone H1 (1 mg in 300 μl of 50 mM NaHCO3) and incubated for 1 hour at 20° C. After dialysis against 5 mM sodium phosphate (pH 6.8) the three samples are stored at -20° C. The concentrations of the biotin-labelled histone H1 solutions are determined by comparison with unmodified histone H1 by SDS-polyacrylamide gel electrophoresis. The number of biotin residues in the three preparations was estimated by the tritium marker contained in BHSE and was found to be between 2.7 and 20 per histone H1 molecule.

후처리

  1. customAfter dialysis against 5 mM sodium phosphate (pH 6.8) the three samples
  2. customare stored at -20° C

출처 및 근거

ORD 기록
ord-5bf0f635159d482cb89e9366520da456
출처 데이터셋
uspto-grants-1991_10
특허 번호
US05053326

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