Sodium Chloride → 5'-Thymidylic acid

HRID 345510

반응식

HRID 345510 반응식
C(O)C(N)(CO)CO.Cl.[Mg+2].[Cl-].[Cl-].[Na+].[Cl-].SC[C@H]([C@@H](CS)O)O.[P:23]([O:35][CH2:36][C@H:37]1[O:41][C@@H:40]([N:42]2[CH:50]=[C:48]([CH3:49])[C:46](=[O:47])[NH:45][C:43]2=[O:44])[CH2:39][C@@H:38]1[OH:51])([O:26][P:27]([O:30][P:31]([OH:34])([OH:33])=[O:32])([OH:29])=[O:28])(=[O:25])[OH:24]>>[CH3:49][C:48]1[C:46](=[O:47])[NH:45][C:43](=[O:44])[N:42]([C@@H:40]2[O:41][C@H:37]([CH2:36][O:35][P:23]([OH:25])([OH:26])=[O:24])[C@@H:38]([OH:51])[CH2:39]2)[CH:50]=1.[P:23]([O:35][CH2:36][C@H:37]1[O:41][C@@H:40]([N:42]2[CH:50]=[C:48]([CH3:49])[C:46](=[O:47])[NH:45][C:43]2=[O:44])[CH2:39][C@@H:38]1[OH:51])([O:26][P:27]([O:30][P:31]([OH:33])([OH:34])=[O:32])([OH:29])=[O:28])(=[O:24])[OH:25]

참가 물질

7

반응물 5

HCID 5234Sodium ChlorideHCID 64968dTTPHCID 93573Tromethamine hydrochlorideHCID 446094D-1,4-DithiothreitolHCID 5360315Magnesium Chloride

생성물 2

HCID 97005'-Thymidylic acidHCID 64968dTTP

실험 절차

The thus prepared solution of the test compound was mixed with a solution containing 50 mM of Tris-HCl buffer (pH 8.3), 8 mM of MgCl2, 30 mM of NaCl, 50 mM of dithiothreitol (made by Wako Pure Chemical Industries Ltd.), 0.2 mM of thymidine-5'-triphosphate (made by Pharmacia K. K.) and 6 U/ml of reverse transcriptase derived from M-MLV (made by Pharmacia K. K.), and preincubated at 37° C. for 30 minutes. After there was added thereto 10 μg/ml of polyadenylic acid (made by PL Biochemicals Co., Ltd.), 0.01 U/ml of oligodeoxy thymidylic acid (made by Pharmacia K. K.) and 10 μCi/ml of [methyl-3H] thymidine-5'-triphosphate (made by Amersham Japan Co., Ltd., 47 Ci/mmol) to give a reaction mixture, the mixture was further incubated at 37° C. for 30 minutes, followed by cooling with ice to stop the reaction.

후처리

  1. additionThe thus prepared solution of the test compound was mixed with a solution
  2. additionAfter there was added

출처 및 근거

ORD 기록
ord-a2e40a2a46274300ac50fb9442098920
출처 데이터셋
uspto-grants-1991_10
특허 번호
US05057538

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