Water → H-Asp-Arg-Val-Tyr-Val-His-Pro-Ala-OH

HRID 358157

반응식

HRID 358157 반응식
OP([O-])([O-])=O.[K+].[K+].OP([O-])(O)=O.[K+].ClC1N=C(Cl)N=C(Cl)N=1.C[CH2:24][C@@H:25]([C@H:27]([NH:77][C:78]([C@@H:80]([NH:89][C:90]([C@@H:92]([NH:96][C:97]([C@@H:99]([NH:107][C:108]([C@@H:110]([NH2:115])[CH2:111][C:112]([OH:114])=[O:113])=[O:109])[CH2:100][CH2:101][CH2:102][N:103]=[C:104]([NH2:106])[NH2:105])=[O:98])[CH:93]([CH3:95])[CH3:94])=[O:91])[CH2:81][C:82]1[CH:87]=[CH:86][C:85]([OH:88])=[CH:84][CH:83]=1)=[O:79])[C:28]([NH:30][C@H:31]([C:38]([N:40]1[C@H:44]([C:45]([NH:47][C@H:48]([C:56](N[C@H](C(N[C@H](C(O)=O)CC(C)C)=O)CC2NC=NC=2)=[O:57])[CH2:49]C2C=CC=CC=2)=[O:46])[CH2:43][CH2:42][CH2:41]1)=[O:39])[CH2:32][C:33]1[NH:37][CH:36]=[N:35][CH:34]=1)=[O:29])[CH3:26].[OH2:116]>O1CCOCC1.CO>[CH3:49][C@H:48]([NH:47][C:45]([C@H:44]1[N:40]([C:38]([C@@H:31]([NH:30][C:28]([C@@H:27]([NH:77][C:78]([C@@H:80]([NH:89][C:90]([C@@H:92]([NH:96][C:97]([C@@H:99]([NH:107][C:108]([C@@H:110]([NH2:115])[CH2:111][C:112]([OH:114])=[O:113])=[O:109])[CH2:100][CH2:101][CH2:102][NH:103][C:104]([NH2:106])=[NH:105])=[O:98])[CH:93]([CH3:94])[CH3:95])=[O:91])[CH2:81][C:82]2[CH:87]=[CH:86][C:85]([OH:88])=[CH:84][CH:83]=2)=[O:79])[CH:25]([CH3:24])[CH3:26])=[O:29])[CH2:32][C:33]2[NH:37][CH:36]=[N:35][CH:34]=2)=[O:39])[CH2:41][CH2:42][CH2:43]1)=[O:46])[C:56]([OH:57])=[O:116]

참가 물질

8

반응물 5

HCID 962Water2회HCID 7954Cyanuric chlorideHCID 24450Potassium Phosphate, DibasicHCID 516951Monobasic potassium phosphateHCID 3081372Angiotensin I

생성물 1

HCID 178448346H-Asp-Arg-Val-Tyr-Val-His-Pro-Ala-OH

시약·촉매·용매 2

HCID 887Methanol용매HCID 312751,4-Dioxane용매

실험 절차

A 25 μl aliquot of the enzyme suspension was added to the above reaction-broth mixture and incubated at 37° C. for 30 minutes. The enzyme reaction was quenched by placing the mixture in a boiling water bath for 10 minutes. A blank was prepared by adding the enzyme aliquot to the reaction mixture after the boiling water treatment. A positive control consisted of a 10 μl mixture of methanol and water (1:1). A 1.0 ml portion of 0.2M K2HPO4 --KH2PO4 buffer (pH=8.3) was added to each boiled mixture and then 0.5 ml of 3% 2,4,6-trichloro-S-triazine in dioxane (3% TT) was added and the mixture vortexed. The solutions were centrifuged and the supernatant from each solution was measured for optical density (O.D.) at 382 nm. The percentage of inhibition of the angiotensin I-converting enzyme was defined by the equation: ##EQU1## Broth samples from medium 3 (see Table I) showing at least 90% inhibition were deemed positive. For the other media listed, a minimum inhibition percentage of 80% indicated a positive result. A positive assay result indicated that the cultured broth was producing the desired compound, 176.

후처리

  1. additionA 25 μl aliquot of the enzyme suspension was added to the above reaction-broth mixture
  2. customThe enzyme reaction
  3. customwas quenched
  4. customfor 10 minutes
  5. customA blank was prepared
  6. customA positive assay result

출처 및 근거

ORD 기록
ord-8442d0f681a74d14986aca25c33ec446
출처 데이터셋
uspto-grants-1984_08
특허 번호
US04464395

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