Paclitaxel → Paclitaxel

HRID 439695

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HRID 439695 반응식
[CH3:1][C:2]1[C@@H:19]([O:20][C:21]([C@H:23]([OH:40])[C@@H:24]([NH:31][C:32]([C:34]2[CH:35]=[CH:36][CH:37]=[CH:38][CH:39]=2)=[O:33])[C:25]2[CH:26]=[CH:27][CH:28]=[CH:29][CH:30]=2)=[O:22])[CH2:18][C@:14]2([OH:41])[C:15]([CH3:17])([CH3:16])[C:3]=1[C@@H:4]([O:59][C:60]([CH3:62])=[O:61])[C:5]([C@@:7]1([CH3:58])[C@H:12]([C@@H:13]2[O:42][C:43]([C:45]2[CH:46]=[CH:47][CH:48]=[CH:49][CH:50]=2)=[O:44])[C@:11]2([O:53][C:54]([CH3:56])=[O:55])[CH2:51][O:52][C@@H:10]2[CH2:9][C@@H:8]1[OH:57])=[O:6]>CO>[CH3:1][C:2]1[C@@H:19]([O:20][C:21]([C@H:23]([OH:40])[C@@H:24]([NH:31][C:32]([C:34]2[CH:39]=[CH:38][CH:37]=[CH:36][CH:35]=2)=[O:33])[C:25]2[CH:26]=[CH:27][CH:28]=[CH:29][CH:30]=2)=[O:22])[CH2:18][C@:14]2([OH:41])[C:15]([CH3:16])([CH3:17])[C:3]=1[C@@H:4]([O:59][C:60]([CH3:62])=[O:61])[C:5]([C@@:7]1([CH3:58])[C@H:12]([C@@H:13]2[O:42][C:43]([C:45]2[CH:50]=[CH:49][CH:48]=[CH:47][CH:46]=2)=[O:44])[C@:11]2([O:53][C:54]([CH3:56])=[O:55])[CH2:51][O:52][C@@H:10]2[CH2:9][C@@H:8]1[OH:57])=[O:6].[CH3:1][C:2]1[C@@H:19]([OH:20])[CH2:18][C@:14]2([OH:41])[C:15]([CH3:16])([CH3:17])[C:3]=1[C@@H:4]([O:59][C:60]([CH3:62])=[O:61])[C:5]([C@@:7]1([CH3:58])[C@H:12]([C@@H:13]2[O:42][C:43]([C:45]2[CH:46]=[CH:47][CH:48]=[CH:49][CH:50]=2)=[O:44])[C@:11]2([O:53][C:54]([CH3:56])=[O:55])[CH2:51][O:52][C@@H:10]2[CH2:9][C@@H:8]1[OH:57])=[O:6]

참가 물질

4

반응물 1

HCID 36314Paclitaxel

생성물 2

HCID 36314PaclitaxelHCID 65366Baccatin Iii

시약·촉매·용매 1

HCID 887Methanol용매

반응 조건

온도
23 °C

실험 절차

0.5 mg taxol in 20 μl methanol was added to 2 ml of each supernatant and the mixtures were stirred for 21 hours at ambient temperature (about 23° C.). The solutions were extracted with 4 ml CH2Cl2. Extracts were dried under N2 at room temperature, redissolved in methanol and analyzed by HPLC (Method 1 described following). Supernatant from strain ATCC 55424 (SC 13910) gave 0.008 mg/ml taxol and 0.163 mg/ml baccatin III (94.9 mol % conversion). Supernatant from strain ATCC 55425 (SC 13911) gave 0.014 mg/ml taxol and 0.166 mg/ml baccatin III (96.7 mol % conversion). (Baccatin III and the cleaved side chain product were identified by LC-MS). ##STR6##

후처리

  1. extractionThe solutions were extracted with 4 ml CH2Cl2
  2. customExtracts were dried under N2 at room temperature
  3. dissolutionredissolved in methanol

출처 및 근거

ORD 기록
ord-e9b6dba824e04373b7f14ab3bb695b2a
출처 데이터셋
uspto-grants-1996_05
특허 번호
US05516676

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