Paclitaxel → Paclitaxel

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0.5 mg taxol in 20 μl methanol was added to 2 ml of each supernatant and the mixtures were stirred for 21 hours at ambient temperature (about 23° C.). The solutions were extracted with 4 ml CH2Cl2. Extracts were dried under N2 at room temperature, redissolved in methanol and analyzed by HPLC (Method 1 described following). Supernatant from strain ATCC 55424 (SC 13910) gave 0.008 mg/ml taxol and 0.163 mg/ml baccatin III (94.9 mol % conversion). Supernatant from strain ATCC 55425 (SC 13911) gave 0.014 mg/ml taxol and 0.166 mg/ml baccatin III (96.7 mol % conversion). (Baccatin III and the cleaved side chain product were identified by LC-MS). ##STR6##

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