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HRID439695

反应详情

EQUATION

反应方程式

HRID 439695 的结构方程式

REACTANTS

反应物

1
结构：CC(=O)O[C@H]1C(=O)[C@@]2(C)[C@H]([C@H](OC(=O)c3ccccc3)[C@]3(O)C[C@H](OC(=O)[C@H](O)[C@@H](NC(=O)c4ccccc4)c4ccccc4)C(C)=C1C3(C)C)[C@]1(OC(C)=O)CO[C@@H]1C[C@@H]2O
HCID36314

Paclitaxel

C47H51NO14

PRODUCTS

生成物

2
结构：CC(=O)O[C@H]1C(=O)[C@@]2(C)[C@H]([C@H](OC(=O)c3ccccc3)[C@]3(O)C[C@H](OC(=O)[C@H](O)[C@@H](NC(=O)c4ccccc4)c4ccccc4)C(C)=C1C3(C)C)[C@]1(OC(C)=O)CO[C@@H]1C[C@@H]2O
HCID36314

Paclitaxel

C47H51NO14

结构：CC(=O)O[C@H]1C(=O)[C@@]2(C)[C@H]([C@H](OC(=O)c3ccccc3)[C@]3(O)C[C@H](O)C(C)=C1C3(C)C)[C@]1(OC(C)=O)CO[C@@H]1C[C@@H]2O
HCID65366

Baccatin III

C31H38O11

AUXILIARIES

试剂、催化剂与溶剂

1
结构：CO
溶剂HCID887

Methanol

CH4O

CONDITIONS

反应条件

温度
23 °C

PROCEDURE

实验过程

0.5 mg taxol in 20 μl methanol was added to 2 ml of each supernatant and the mixtures were stirred for 21 hours at ambient temperature (about 23° C.). The solutions were extracted with 4 ml CH2Cl2. Extracts were dried under N2 at room temperature, redissolved in methanol and analyzed by HPLC (Method 1 described following). Supernatant from strain ATCC 55424 (SC 13910) gave 0.008 mg/ml taxol and 0.163 mg/ml baccatin III (94.9 mol % conversion). Supernatant from strain ATCC 55425 (SC 13911) gave 0.014 mg/ml taxol and 0.166 mg/ml baccatin III (96.7 mol % conversion). (Baccatin III and the cleaved side chain product were identified by LC-MS). ##STR6##

WORKUP

后处理

  1. extractionThe solutions were extracted with 4 ml CH2Cl2
  2. customExtracts were dried under N2 at room temperature
  3. dissolutionredissolved in methanol