Iodine → Beta-D-Glucose 6-phosphate

HRID 441477노트

반응식

HRID 441477 반응식
C(=O)(O)[O-].[Na+].[CH2:6]([O:17][P:18]([OH:21])([OH:20])=[O:19])[CH:7]([OH:16])[CH:8]([OH:15])[CH:9]([OH:14])[CH:10]([OH:13])[CH:11]=[O:12].[Ba].C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1.II>CN(C=O)C>[CH2:6]([O:17][P:18]([OH:21])([OH:20])=[O:19])[C@H:7]1[O:16][C@@H:11]([OH:12])[C@H:10]([OH:13])[C@@H:9]([OH:14])[C@@H:8]1[OH:15]

참가 물질

6

반응물 4

HCID 807IodineHCID 439153NadhHCID 516892Sodium BicarbonateHCID 16219436Barium--6-O-phosphonohexose (1/1)

생성물 1

HCID 439427Beta-D-Glucose 6-phosphate

시약·촉매·용매 1

HCID 6228Dimethylformamide용매3회

실험 절차

Following the procedure described above, 10mg of the T4 methyl ester diglycolate in 0.52ml dry DMF was reacted with 1.3mg NHS and 2.3mg ECDI. To 8.7mg G-6-PDH (0.08μ mole) in 1.65ml 1mg/ml sodium bicarbonate was added 5mg G-6-P and 12.5mg NADH, followed by 0.335ml DMF, followed by 9.5μmole ester prepared above in 0.215ml DMF. In 4 hours, a 47% loss in activity was observed. The hapten number was found to be 12 by UV and 13.2 by iodine analysis.

후처리

  1. customwas reacted with 1.3mg NHS and 2.3mg ECDI

출처 및 근거

ORD 기록
ord-c08a2a382b3b4e68af4b574d4ed770bf
출처 데이터셋
uspto-grants-1977_08
특허 번호
US04040907

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