Ethanol → Ninhydrin

HRID 485081노트

반응식

HRID 485081 반응식
C1N[C@@H](C(O)=O)CN([CH2:10][CH2:11][CH2:12][C:13]([OH:15])=[O:14])C1.[CH3:16][CH2:17][OH:18].[CH2:19](O)[CH2:20][CH2:21]C.C(O)(=[O:26])C.O>>[CH:19]1[CH:10]=[C:11]2[C:12]([C:13]([OH:14])([OH:15])[C:17](=[O:18])[C:16]2=[CH:21][CH:20]=1)=[O:26]

참가 물질

4

반응물 3

HCID 702EthanolHCID 22056973Butanol acetic acid waterHCID 44277691HCID 44277691, 화합물

생성물 1

HCID 10236Ninhydrin

실험 절차

The column is washed with 80 l of water, followed by passage of 240 l of 0-10% EtOH at SV=1.0 (80 l/hr.). The eluate is collected in 8 l fractions which are assayed by HPLC. The HPLC gives an elution curve corresponding to the first half of the attached drawing. It is clear that D-CPC and DA-CPC are eluted in two distinct patterns. A further amount of 10% EtOH may be passed to elute CPC but in order to save on the elution time and obtain a better-defined elution curve, the eluant is replaced with 20% EtOH and the eluate is collected in 8 l fractions. As assayed by HPLC, there is obtained an elution curve corresponding to the second half of the drawing. Each fraction is applied to a thin-layer chromatoplate of microcrystalline cellulose powder (Tokyo Kasei K.K. Japan) and the chromatogram is developed with n-butanol-acetic acid-water (3:1:1, V/V), and dried in air. The image formed by color-reaction with ninhydrin shows a spot each at Rf=0.15 for D-CPC, RF=0.28 for CPC and Rf=0.30 for DA-CPC.

후처리

  1. washThe column is washed with 80 l of water
  2. customThe eluate is collected in 8 l fractions which
  3. customThe HPLC gives an elution curve corresponding to the first half of the attached drawing
  4. washare eluted in two distinct patterns
  5. washto elute CPC
  6. customobtain a better-defined elution curve
  7. customthe eluate is collected in 8 l fractions
  8. customthere is obtained an elution curve corresponding to the second half of the drawing
  9. customdried in air

출처 및 근거

ORD 기록
ord-4573794d7611458084fec677ad581975
출처 데이터셋
uspto-grants-1985_08
특허 번호
US04535155

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