Bistris → Sodium Lauryl Sulfate

HRID 514587

반응식

HRID 514587 반응식
C(N(C(CO)(CO)CO)CCO)CO.C1N(CCS(O)(=O)=O)CCOC1.[CH3:27][CH2:28][CH2:29][CH2:30][CH2:31][CH2:32][CH2:33][CH2:34][CH2:35][CH2:36][CH2:37][CH2:38][O:39][S:40]([O-:43])(=[O:42])=[O:41].[Na+:44]>O>[CH3:27][CH2:28][CH2:29][CH2:30][CH2:31][CH2:32][CH2:33][CH2:34][CH2:35][CH2:36][CH2:37][CH2:38][O:39][S:40]([O-:43])(=[O:42])=[O:41].[Na+:44]

참가 물질

4

반응물 2

HCID 81462BistrisHCID 86623582Mes sds

생성물 1

HCID 3423265Sodium Lauryl Sulfate

시약·촉매·용매 1

HCID 962Water용매

반응 조건

온도
95 °C

실험 절차

Samples were diluted to 1 mg/ml with the relevant buffer and 16.71a1 added to 12.5 μl of 4×LDS sample buffer (Invitrogen), 15.8 μl of Milli-Q water and 5 μl reducing agent (Invitrogen). The samples were heated at 95° C. for one minute and then placed on ice. 15 μl of each sample was loaded onto a 4-12% BisTris gel (Invitrogen) in a tank containing 1×MES SDS running buffer and the gel run for 35 minutes at a constant current of 500 mA. After electrophoresis the gel was removed from its casing, rinsed for 3×10 minutes with Milli-Q water, stained with Gelcode® Blue staining reagent (Pierce) for a minimum of one hour and then destained with Milli-Q water. The gel was photographed and analysed using a UVP GDS8000 gel documentation system. The relative abundance of BAK502G9 heavy and light chain in each sample was determined.

후처리

  1. additionSamples were diluted to 1 mg/ml with the relevant buffer and 16.71a1
  2. customwas removed from its casing
  3. washrinsed for 3×10 minutes with Milli-Q water
  4. customstained with Gelcode® Blue staining reagent (Pierce) for a minimum of one hour

출처 및 근거

ORD 기록
ord-f744a55c73fb4174a5c2be4196d67f6b
출처 데이터셋
uspto-grants-2015_08
특허 번호
US09107945B2

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