Cholesterol → Cholesteryl Oleate

HRID 582223

반응식

HRID 582223 반응식
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[CH3:13][CH:14]([CH2:16][CH2:17][CH2:18][C@H:19]([C@@H:21]1[C@:39]2([CH3:40])[C@H:24]([C@H:25]3[C@H:36]([CH2:37][CH2:38]2)[C@:34]2([CH3:35])[C:28]([CH2:29][C@H:30]([CH2:32][CH2:33]2)[OH:31])=[CH:27][CH2:26]3)[CH2:23][CH2:22]1)[CH3:20])[CH3:15].[OH:41][C:42]([CH2:45][CH2:46][CH2:47][C@H:48]([C@@H:50]1[C@]2(C)[C@H:53]([C@H:54]3[C@H](CC2)[C@:63]2(C)[C:57]([CH2:58][C@H:59]([CH2:61][CH2:62]2)O)=[CH:56][CH2:55]3)[CH2:52][CH2:51]1)C)(C)C.S([O-])(OCCCCCCCCCCCC)(=O)=O.[Na+]>>[CH3:63][CH2:62][CH2:61][CH2:59][CH2:58][CH2:57][CH2:56][CH2:55]/[CH:54]=[CH:53]\[CH2:52][CH2:51][CH2:50][CH2:48][CH2:47][CH2:46][CH2:45][C:42]([O:31][C@@H:30]1[CH2:29][C:28]2[C@@:34]([CH3:35])([C@@H:36]3[C@@H:25]([CH2:26][CH:27]=2)[C@@H:24]2[CH2:23][CH2:22][C@H:21]([C@@H:19]([CH2:18][CH2:17][CH2:16][CH:14]([CH3:13])[CH3:15])[CH3:20])[C@@:39]2([CH3:40])[CH2:38][CH2:37]3)[CH2:33][CH2:32]1)=[O:41]

참가 물질

5

반응물 4

HCID 5997CholesterolHCID 6509425-HydroxycholesterolHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 3423265Sodium Lauryl Sulfate

생성물 1

HCID 5283632Cholesteryl Oleate

실험 절차

More specifically, cultured J774 cells were plated on a 24 F culture plate containing a serum-free RPMI 1640 medium so as to account for 1×10-6 cells/well. Added to the cells were [14C]oleic acid, delipidized BAS (bovine serum albumin), reconstituted ribosome (0.3 M glucose solution containing cholesterol and phosphatidylcholine at a weight ratio of 2:1) and 25-hydroxycholesterol to culture the cells for 4 hours at 37° C. under 5% CO2. After the culturing, the cultured cells were disrupted with a 1% SDS (sodium dodecyl sulfate) solution, and the lipid in the disrupted solution was extracted with hexane. The extract was dried to solid under reduced pressure. After the resultant residue was then developed (developing solvent: diethyl ether/hexane/acetic acid=80/20/1) by TLC (thin-layer chromatography), the amount of cholesteryl oleate formed was determined by an imaging plate.

후처리

  1. additioncontaining a serum-free RPMI 1640 medium so as
  2. additionAdded to the cells
  3. extractionthe lipid in the disrupted solution was extracted with hexane
  4. customThe extract was dried to solid under reduced pressure

출처 및 근거

ORD 기록
ord-7314d84312fb4cb6a848911c9ee9c33f
출처 데이터셋
uspto-grants-1999_07
특허 번호
US05922767

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