Water → Deoxyuridine Triphosphate
반응식
O.[P:2]([O:14][CH2:15][C@H:16]1[O:20][C@@H:19]([N:21]2[CH:28]=[C:27](CC=CN)[C:25](=[O:26])[NH:24][C:22]2=[O:23])[CH2:18][C@@H:17]1[OH:33])([O:5][P:6]([O:9][P:10]([OH:13])([OH:12])=[O:11])([OH:8])=[O:7])(=[O:4])[OH:3]>CS(C)=O.C(=O)([O-])[O-].[Na+].[Na+].C(=O)(O)[O-]>[P:2]([O:14][CH2:15][C@H:16]1[O:20][C@@H:19]([N:21]2[CH:28]=[CH:27][C:25](=[O:26])[NH:24][C:22]2=[O:23])[CH2:18][C@@H:17]1[OH:33])([O:5][P:6]([O:9][P:10]([OH:12])([OH:13])=[O:11])([OH:8])=[O:7])(=[O:3])[OH:4]참가 물질
5
실험 절차
Dye-PC NHS ester (3 equivalents) dissolved in DMSO or other water miscible solvent is added to a solution of 5-(3-aminoallyl)-2′-deoxyuridine-5′-triphosphate in 0.1M sodium carbonate/bicarbonate buffer (pH 8.5). Mixture is stirred at room temperature for 2-3 h and crude reaction is purified by reversed-phase HPLC.