반응식
CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O.C[C@H]1[C@@H](CC2OC3C=CC(NC)=C(C(O)=O)C=3N=2)O[C@]2(O[C@H]([C@@H](C(C3NC=CC=3)=O)C)[C@H](C)C[C@H]2C)CC1.C(O)(=O)CC(CC(O)=O)(C(O)=O)O.CC(C(CC1C=CC(O)=C(O)C=1)C)CC1C=CC(O)=C(O)C=1.[CH3:96][CH2:97][CH2:98][CH2:99][CH2:100][CH:101]=[CH:102][CH2:103][CH:104]=[CH:105][CH2:106][CH:107]=[CH:108][CH:109]=[CH:110][CH:111]([OH:118])[CH2:112][CH2:113][CH2:114][C:115]([OH:117])=[O:116]>>[CH3:96][CH2:97][CH2:98][CH2:99][CH2:100]/[CH:101]=[CH:102]\[CH2:103]/[CH:104]=[CH:105]\[CH2:106]/[CH:107]=[CH:108]\[CH:109]=[CH:110]\[C@@H:111]([OH:118])[CH2:112][CH2:113][CH2:114][C:115]([OH:117])=[O:116]참가 물질
6
실험 절차
A suspension of human neutrophils in buffer is incubated for 3 min at 30° C. with (14C)-arachidonic acid (AA) and calcium ionophore A23187. Citric Acid (2M) /NDGA* (10 mM) is used to quench the reaction. Following the addition of a trace amount of (3H)-5-HETE together with an excess of unlabeled 5-HETE to each tube, the mixture is extracted with chloroform/methanol. The organic layer is washed with dilute hydrochloric acid and the total volume is transferred to glass tubes and dried in vacuo. The residue is dissolved in a small volume of chloroform and is spotted on silica gel TLC sheets which are developed with an ethyl acetate/isooctane/water/acetic acid solvent system
후처리
- additionA suspension of human neutrophils
- customto quench
- customthe reaction
- extractionthe mixture is extracted with chloroform/methanol
- washThe organic layer is washed with dilute hydrochloric acid
- customdried in vacuo
- dissolutionThe residue is dissolved in a small volume of chloroform
출처 및 근거
- ORD 기록
- ord-9d92797f31cb495c9f7ebc60d4534c5c
- 출처 데이터셋
- uspto-grants-1987_06
- 특허 번호
- US04677210
연결된 노트
불러오는 중…