L-Glutamine → L-Alanyl-L-Glutamine

HRID 659927

반응식

HRID 659927 반응식
B([O-])([O-])[O-].C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O.Cl.C[O:27][C:28](=O)[C@H:29]([CH3:31])[NH2:30].[NH2:33][C@H:34]([C:40]([OH:42])=[O:41])[CH2:35][CH2:36][C:37](=[O:39])[NH2:38]>>[NH2:30][C@H:29]([C:28]([NH:33][C@H:34]([C:40]([OH:42])=[O:41])[CH2:35][CH2:36][C:37](=[O:39])[NH2:38])=[O:27])[CH3:31]

참가 물질

5

반응물 4

HCID 5961L-GlutamineHCID 6049Ethylenediaminetetraacetic AcidHCID 26574BorateHCID 2733257Alanine methyl ester hydrochloride

생성물 1

HCID 123935L-Alanyl-L-Glutamine

실험 절차

Each strain of the microbial cells thus obtained was recovered from the agar media, and suspended in a 0.1 M borate buffer (pH 9.0) containing 10 mM EDTA so as to prepare a suspension containing 100 g/l of wet microbial cells. 0.1 ml of each microbial cell suspension was admixed with 0.1 ml of 100 mM borate buffer (pH 9.0) containing 10 mM EDTA, 200 mM L-alanine methyl ester hydrochloride and 400 mM L-glutamine to prepare a total volume 0.2 ml of the reaction mixture, which was then subjected to the reaction at 20° C. for the time period shown in Table 1. The amount (mM) of L-alanyl-L-glutamine (Ala-Gin) produced by each reaction is shown in Table 1.

후처리

  1. customEach strain of the microbial cells thus obtained
  2. customwas recovered from the agar media
  3. additioncontaining 10 mM EDTA so as
  4. customto prepare a suspension
  5. additioncontaining 100 g/l of wet microbial cells
  6. customto prepare a total volume 0.2 ml of the reaction mixture, which
  7. customwas then subjected to the reaction at 20° C. for the time period

출처 및 근거

ORD 기록
ord-2dcdcaae011e43918232a6dec5eab105
출처 데이터셋
uspto-grants-2011_12
특허 번호
US08084227B2

연결된 노트

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