Phosphoric Acid → (2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

HRID 727766노트

반응식

HRID 727766 반응식
[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[P:9](=[O:13])([OH:12])([OH:11])[OH:10]>O>[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[OH:11][P:9]([OH:13])([OH:12])=[O:10]

참가 물질

4

반응물 2

HCID 1004Phosphoric AcidHCID 6503Tris(hydroxymethyl)aminomethane

생성물 1

HCID 3083992(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

시약·촉매·용매 1

HCID 962Water용매2회

실험 절차

A sample of well agitated fermentation mixture (5.0 ml) is centrifuged for five minutes at 17,000 rpm in a centrifuge tube, the supernate decanted and discarded, and the pellet resuspended in 4.9 ml of 0.1M tris phosphate buffer. The tris phosphate buffer is prepared by dissolving 12.1 grams of Sigma Chem. Co. T-1503 Trizma base in 800 ml of deionized water, adjusting the pH at 25° C. to 7.5 with phosphoric acid and diluting with deionized water to a final volume of 1000 ml.

후처리

  1. customthe supernate decanted
  2. dissolutionby dissolving 12.1 grams of Sigma Chem
  3. customat 25° C.

출처 및 근거

ORD 기록
ord-5b9c7780e36b4c9391c0eaef9d762d50
출처 데이터셋
uspto-grants-1991_09
특허 번호
US05045465

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