Bicarbonate Ion → Biotin

HRID 7341

반응식

HRID 7341 반응식
[C:1](=[O:4])(O)[O-:2].[CH2:5]1[S:9][C@@H:8]([CH2:10][CH2:11][CH2:12][CH2:13]C(NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=O)[C@H:7]2[NH:36][C:37]([NH:39][C@@H:6]12)=[O:38].[Na+]>O>[OH:2][C:1]([CH2:13][CH2:12][CH2:11][CH2:10][C@H:8]1[C@@H:7]2[C@@H:6]([NH:39][C:37]([NH:36]2)=[O:38])[CH2:5][S:9]1)=[O:4]

참가 물질

4

반응물 2

HCID 769Bicarbonate IonHCID 71571497Sulphosuccinimidyl-6-(biotinamido)hexanoate

생성물 1

HCID 171548Biotin

시약·촉매·용매 1

HCID 962Water용매

실험 절차

About 2.0 mg of DBP is buffer exchanged with 20 mM bicarbonate at pH 9.6 three times to give a final volume of 1.0 mL. Two aliquots of sulfo-NHS-LC-biotin (Pierce) are dissolved in water to give a 1 mg/mL solution just prior to addition. The first aliquot (10 uL) is added and the reaction is maintained at room temperature for 5 minutes. The second aliquot (10 uL) is added and the reaction proceeds for an additional 4 minutes. The unreacted biotin is removed by size exclusion chromatography using SEPHADEX G-25 and 100 mM PBS, pH 7.4 as the mobile phase.

후처리

  1. customto give a final volume of 1.0 mL
  2. customto give a 1 mg/mL solution just
  3. additionto addition
  4. additionThe first aliquot (10 uL) is added
  5. additionThe second aliquot (10 uL) is added
  6. customThe unreacted biotin is removed by size exclusion chromatography

출처 및 근거

ORD 기록
ord-34edcaa7e3ba4173928e329654c5e7d9
출처 데이터셋
uspto-grants-2006_08
특허 번호
US07087395B1

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