Acetic Acid → L-Ornithine, acetate (1:1)

HRID 742183

반응식

HRID 742183 반응식
[O:1]=[C:2]1[O:8][C@H]([C@H](CO)O)C(O)=[C:3]1O.[NH2:13][C@H:14]([C:22]([OH:24])=[O:23])[CH2:15][CH2:16][CH2:17][NH:18]C(=N)N.C(O)(=O)C>O.S([O-])([O-])(=O)=O.[Mn+2].O>[C:2]([OH:8])(=[O:1])[CH3:3].[NH2:13][C@H:14]([C:22]([OH:24])=[O:23])[CH2:15][CH2:16][CH2:17][NH2:18]

참가 물질

6

반응물 3

HCID 176Acetic AcidHCID 54670067L-Ascorbic AcidHCID 178418600HCID 178418600, 화합물

생성물 1

HCID 6453938L-Ornithine, acetate (1:1)

시약·촉매·용매 2

HCID 177577Manganese sulfate monohydrate촉매HCID 962Water용매

실험 절차

42.3 mg manganese sulfate hydrate (2.5×10-4 molar), 21.8 mg ascorbic acid (1.25×10-4 molar) and 15000 units calf's liver arginase of the Boehringer Mannheim company were added to 1 liter of a 0.75 molar L-arginine solution. After 48 hours the conversion was 100% according to HPLC. The mixture was adjusted with acetic acid to pH 6.8, three quarters of the water drawn off and the ornithine acetate precipitated with one liter ethanol at room temperature. After 15 min. of postagitation the precipitate was filtered off, postwashed with ethanol and dried in a vacuum (40 mbars). The yield of pure ornithine acetate was 137.0 g, 95% of theory). Amount of rotation (c=5 in water)+10.0° (theoretical: +9.0 to +11.0).

후처리

  1. customthe ornithine acetate precipitated with one liter ethanol at room temperature
  2. filtrationAfter 15 min. of postagitation the precipitate was filtered off
  3. customdried in a vacuum (40 mbars)
  4. customAmount of rotation (c=5 in water)+10.0° (theoretical: +9.0 to +11.0)

출처 및 근거

ORD 기록
ord-6afb7c5b38e84213b013130b0ea89f0f
출처 데이터셋
uspto-grants-1996_09
특허 번호
US05554518

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