Boc-D-phenylalanine → 3(R,S)-[2(R)-(tert-butyloxycarbonyl)amino-3-phenylpropionylamino]-5-cycloheptyl-1,3-dihydro-1-methyl-2H-1,4-benzodiazepin-2-one

To a solution of 3(R,S)-amino-5-cycloheptyl-1,3-dihydro-1-methyl-2H-1,4-benzodiazepin-2-one (4.08 g) in anhydrous dimethyl formamide (30 ml), under an atmosphere of nitrogen, was added in succession Boc-D-phenylalanine (3.98 g), 1-hydroxybenzotriazole trihydrate (2.03 g) and 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide hydrochloride (2.88 g). Triethylamine (2.09 ml) was then added and the resulting suspension stirred at ambient temperature for 1 h. The solvent was removed under reduced pressure and the residue partitioned between ethyl acetate (100 ml) and 10% citric acid solution (100 ml). The organic phase was separated and the aqueous phase extracted with ethyl acetate (3×50 ml). The combined organic phases were washed with 10% sodium hydroxide solution (100 ml), water (50 ml) and brine (50 ml). The organic layer was separated, dried (Na2SO4) and evaporated in vacuo to afford crude 3(R,S)-[2(R)-(tert-butyloxycarbonyl)amino-3-phenylpropionylamino]-5-cycloheptyl-1,3-dihydro-1-methyl-2H-1,4-benzodiazepin-2-one.

1. customThe solvent was removed under reduced pressure
2. customthe residue partitioned between ethyl acetate (100 ml) and 10% citric acid solution (100 ml)
3. customThe organic phase was separated
4. extractionthe aqueous phase extracted with ethyl acetate (3×50 ml)
5. washThe combined organic phases were washed with 10% sodium hydroxide solution (100 ml), water (50 ml) and brine (50 ml)
6. customThe organic layer was separated
7. dry with materialdried (Na2SO4)
8. customevaporated in vacuo

ORD 기록

ord-b125c3d00749400c8380277136f57c88

출처 데이터셋

uspto-grants-1996_09

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US05556969