3-Cyanopyridine → Nicotinamide

HRID 750433

반응식

HRID 750433 반응식
P([O-])([O-])([O-])=O.[K+].[K+].[K+].[C:9]([C:11]1[CH:12]=[N:13][CH:14]=[CH:15][CH:16]=1)#[N:10].C(#[N:19])C.[OH2:20].CN(C)[C:23](=[O:30])[C:24]1[CH:29]=[CH:28][CH:27]=C[CH:25]=1>C(#N)C>[C:9]([NH2:10])(=[O:30])[C:11]1[CH:16]=[CH:15][CH:14]=[N:13][CH:12]=1.[C:23]([OH:30])(=[O:20])[C:24]1[CH:29]=[CH:28][CH:27]=[N:19][CH:25]=1

참가 물질

7

반응물 4

HCID 793-Cyanopyridine3회HCID 11916N,N-DimethylbenzamideHCID 62657Potassium Phosphate, TribasicHCID 18373917Acetonitrile--water (1/1)

생성물 2

HCID 936NicotinamideHCID 938Nicotinic Acid

시약·촉매·용매 1

HCID 6342Acetonitrile용매

실험 절차

To a 15-mL polypropylene centrifuge tube was added 3.73 mL of 50 mM potassium phosphate buffer (pH 7.0), 1.0 mL of an E. coli SW132 cell extract suspension prepared as described above, and 0.2626 g of 3-cyanopyridine. The final concentration of 3-cyanopyridine was 0.494 M. The reaction mixture was mixed on a rotating platform at 23° C. After 15 min, 7.50 mL of 95:5 acetonitrile/water containing 0.30 M N,N-dimethylbenzamide (HPLC external standard) was added to the reaction, the resulting mixture centrifuged, and a 0.100 mL of the supernatant mixed with 0.900 mL of acetonitrile and analyzed by HPLC. The conversion of 3-cyanopyridine was 100%, and the yields of nicotinamide and nicotinic acid were 100% and 0%, respectively.

후처리

  1. extractioncell extract suspension
  2. customprepared
  3. additionThe reaction mixture was mixed on a rotating platform at 23° C

출처 및 근거

ORD 기록
ord-214a51ab1cce49e8929b5e1ed98a6808
출처 데이터셋
uspto-grants-2008_07
특허 번호
US07405064B2

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