Diisopropylethylamine → 4'-{6-Butyl-3-[(2-mercaptomethyl-4-methylpentanoylamino)methyl]-2-oxo-2h-pyridin-1-ylmethyl}biphenyl-2-carboxylic acid

2-Acetylsulfanylmethyl-4-methyl-pentanoic acid (24 mg, 1.1 eq) and HATU (44 mg, 1.1 eq) were dissolved in DMF (2 mL). DIPEA (740 μL, 4 eq) was added, and the mixture was stirred at room temperature for 15 minutes. 4′-(3-Aminomethyl-6-butyl-2-oxo-2H-pyridin-1-ylmethyl)biphenyl-2-carboxylic acid HCl (45 mg, 0.1 mmol) was added, and the solution was stirred at room temperature for 4 hours. The reaction was quenched with water (8 mL) and concentrated HCl (3 drops), and the mixture extracted with EtOAc (10 mL). The organic layer was dried over Na2SO4, and evaporated. The residue was dissolved in MeOH (1 mL) and 1M NaOH (3 mL). The solution was stirred under nitrogen for 20 minutes, and the reaction was then quenched with acetic acid (2 mL). The MeOH was evaporated, and the aqueous component extracted with EtOAc (10 mL). The solvent was evaporated and the residue purified by reverse phase chromatography to afford the title compound (7.5 mg, 140 μmol). MS m/z: [M+H+] calcd for C31H39N2O4S, 535.7. found 535.2.

1. stirringthe solution was stirred at room temperature for 4 hours
2. customThe reaction was quenched with water (8 mL) and concentrated HCl (3 drops)
3. extractionthe mixture extracted with EtOAc (10 mL)
4. dry with materialThe organic layer was dried over Na2SO4
5. customevaporated
6. dissolutionThe residue was dissolved in MeOH (1 mL)
7. stirringThe solution was stirred under nitrogen for 20 minutes
8. customthe reaction was then quenched with acetic acid (2 mL)
9. customThe MeOH was evaporated
10. extractionthe aqueous component extracted with EtOAc (10 mL)
11. customThe solvent was evaporated
12. customthe residue purified by reverse phase chromatography

ORD 기록

ord-f1dfa900ce234abeb21e7de6b36bf212

출처 데이터셋

uspto-grants-2016_03

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US09273008B2