5'-Atp → Adenosine Monophosphate

HRID 965471

반응식

[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4]P(O)(O)=O)(=[O:3])[OH:2].P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.OP([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+].C(O)C(N)(CO)CO.Cl.[Mg]>>[CH:23]1[N:22]=[C:20]([NH2:21])[C:19]2[N:18]=[CH:17][N:16]([C@@H:14]3[O:15][C@H:11]([CH2:10][O:9][P:1]([OH:4])([OH:3])=[O:2])[C@@H:12]([OH:27])[C@H:13]3[OH:26])[C:25]=2[N:24]=1

참가 물질

반응물 5

HCID 59575'-Atp

HCID 60225'-Adp2회

HCID 93573Tromethamine hydrochloride

HCID 5462224Magnesium

HCID 24978514Phosphate Buffered Saline

생성물 1

HCID 6083Adenosine Monophosphate

실험 절차

A second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow is included for feeding very pure adenosine diphosphate (ADP) reagent, with respect to ATP, into the processing fluid downstream of the junction (5) where detergent is added, but upstream of the luminometer. The ADP reagent (99.95% pure with respect to ATP) is added at a flow and concentration such as to produce a concentration of 0.5 mM in the final flow. The processing fluid is pH 7.4 phosphate buffered saline or TrisHCl pH 7.8 including 0.2 mM magnesium ions.

후처리

customA second embodiment of the luminometer of the present invention is provided wherein an additional reagent flow
customto produce a concentration of 0.5 mM in the final flow

출처 및 근거

ORD 기록: ord-f46e2aa47c954c5db35f79c7548b9286
출처 데이터셋: uspto-grants-1998_06
DOI: 10.6084/m9.figshare.5104873.v1
특허 번호: US05773710

연결된 노트

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