(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → beta-Maltose

HRID 981011

반응식

HRID 981011 반응식
[CH2:1]([OH:45])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]([O:17][C@H]3[C@H](O)[C@@H](O)[C@@H](O[C@H]4[C@H](O)[C@@H](O)[C@@H](O)O[C@@H]4CO)O[C@@H]3CO)[O:11][C@@H:10]2[CH2:40][OH:41])[C@H:5]([OH:42])[C@@H:4]([OH:43])[C@@H:3]1[OH:44].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>>[CH2:1]([OH:45])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])[O:11][C@@H:10]2[CH2:40][OH:41])[C@H:5]([OH:42])[C@@H:4]([OH:43])[C@@H:3]1[OH:44]

참가 물질

3

반응물 2

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 446495Alpha-maltotetraose4회

생성물 1

HCID 6255beta-Maltose

실험 절차

Maltotetraose is the preferred α(1→4) linked glucan for use in the present invention. Maltotetraose is an oligosaccharide consisting of four α(1→4) linked glucose units and is commercially available from Sigma Chemical Company, St. Louis, Missouri, or Boehringer Mannheim, GmbH, Mannheim, West Germany. Where maltotetraose is employed as the α(1→4) linked glucan, the kinetic assay proceeds as follows: Maltotetraose substrate is hydrolyzed by α-amylase in the test sample to yield two moles of maltose per mole of substrate. The enzyme maltose phosphorylase (MP) catalyzes the phosphorolysis of each mole of maltose to one mole each of glucose and β-D-glucose-1-phosphate. The enzyme β-phosphoglucomutase (β-PGM) converts the β-glucose-1-phosphate to glucose-6-phosphate which is then oxidized to 6-phosphogluconate with the concomitant reduction of nicotinamide adenine dinucleotide (NAD) to NADH in the reaction catalyzed by G-6-PDH.

출처 및 근거

ORD 기록
ord-0d4e50c39d4f4a61a99705fec6be2718
출처 데이터셋
uspto-grants-1991_02
특허 번호
US04990445

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