Dimethyl Sulfate → HCID 178465533, 화합물

HRID 986078

반응식

HRID 986078 반응식
[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[C:30]3[N:29]=[C:27]([NH2:28])[NH:26][C:24](=[O:25])[C:23]=3[N:22]=[CH:21]2)[C@H:17]([OH:31])[C@@H:16]1[OH:32])([O:4][P:5]([O:8][P:9]([OH:12])([OH:11])=[O:10])([OH:7])=[O:6])(=[O:3])[OH:2].S(OC)(O[CH3:37])(=O)=O>>[CH3:37][N:22]1[C:23]2[C:24]([N:26]=[C:27]([NH2:28])[NH:29][C:30]=2[N+:20]([C@@H:18]2[O:19][C@H:15]([CH2:14][O:13][P:1]([O:4][P:5]([O:8][P:9]([OH:11])([OH:12])=[O:10])([OH:7])=[O:6])([OH:2])=[O:3])[C@@H:16]([OH:32])[C@H:17]2[OH:31])=[CH:21]1)=[O:25].[CH2:30]([NH+:20]([CH2:18][CH3:17])[CH2:21][CH3:37])[CH3:23]

참가 물질

3

반응물 2

HCID 6497Dimethyl SulfateHCID 135398633Guanosine Triphosphate

생성물 1

HCID 178465533HCID 178465533, 화합물

실험 절차

Guanosine triphosphate (GTP) (300 mg, 0.5 mmol) was methylated using dimethyl sulfate according to the procedure of Hendler et al. ((1970) Biochemistry 9, 414) with modification. The reaction mixture was neutralized and the product purified on a DEAE Sephadex A-25 (Sephadex is a registered trademark of Pharmacia Fine Chemicals, Inc.) column (3×50 cm). The column was washed with water and then eluted with a linear gradient of 0 to 0.8M triethylammonium bicarbonate (TEAB), pH 7.8 (2 l total volume). Twenty-milliliter fractions were collected and assayed spectrophometrically at 260 nm. m7GTP eluted at approximately 0.4M, and the peak was pooled and repeatedly evaporated to dryness from water vacuo. m7GTP-triethylammonium salt was obtained as a white solid (0.25 mmol, 50% yield).

후처리

  1. customthe product purified on a DEAE Sephadex A-25 (Sephadex
  2. washThe column was washed with water
  3. washeluted with a linear gradient of 0 to 0.8M triethylammonium bicarbonate (TEAB), pH 7.8 (2 l total volume)
  4. customTwenty-milliliter fractions were collected
  5. washm7GTP eluted at approximately 0.4M
  6. customrepeatedly evaporated to dryness from water vacuo

출처 및 근거

ORD 기록
ord-0bbd8c4c618047118d9ee8b27e01bd24
출처 데이터셋
uspto-grants-1987_12
특허 번호
US04710566

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