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HRID1012889

反应详情

EQUATION

反应方程式

HRID 1012889 的结构方程式

REACTANTS

反应物

7
结构：O=C=O
HCID280

Carbon dioxide

CO2

结构：CCC(=O)OC1(c2ccccc2)CCN(C)CC1
HCID61583

Desmethylprodine

C15H21NO2

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
HCID107526

未命名化合物

结构：CC(=O)C(=O)[O-]
HCID107735

Pyruvate

C3H3O3-

结构：C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)O
HCID123935

Alanylglutamine

C8H15N3O4

结构：CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]
HCID23664709

Penicillin G potassium

C16H17KN2O4S

结构：CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
HCID178484872

未命名化合物

PRODUCTS

生成物

1
结构：CN[C@H]1[C@@H](O[C@@H]2/C3=C/C#C[C@H]4O[C@@]4([C@H]4COC(=O)O4)C#CC3=C[C@H]2OC(=O)c2cccc3c(C)cc(OC)cc23)O[C@H](C)[C@H](O)[C@@H]1O
HCID44581473

未命名化合物

PROCEDURE

实验过程

Human cervix adenocarcinoma HeLa cells are maintained as exponentially growing monolayers in Dulbecco modified Eagle's minimum essential medium with 4.5 g/l glucose, 0.1 g/l pyruvate, 105 Ul/l penicilline, 0.1 g/l streptomycine, 0.86 g/l Glutamax I and 10% v/v foetal calf serum (37° C., 5% CO2), without or with 5 μM DL-threo-1-phenyl-2-hexadecanoyl-amino-3-morpholino-1-propanol (PPMP) to inhibit Gb3 expression. When present, PPMP is introduced into pre-cultures for at least 6 days prior to cytotoxicity assays and is present for up to drug wash.

WORKUP

后处理

  1. temperatureHuman cervix adenocarcinoma HeLa cells are maintained as exponentially growing monolayers in Dulbecco
  2. washwash