L-Phenylalanine → (2S)-2-(fluoroamino)-3-phenylpropanoic acid

HRID 1014910Notes

Reaction equation

HRID 1014910 reaction equation
[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][C:4]1[CH:9]=[CH:8][CH:7]=[CH:6][CH:5]=1.[F:13]C1C=CC(C[C@@H](C(O)=O)N)=CC=1.N[C@H](C(O)=O)CC[Se]C>>[F:13][NH:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][C:4]1[CH:9]=[CH:8][CH:7]=[CH:6][CH:5]=1

Participants

4

Reactant 3

HCID 6140L-PhenylalanineHCID 105024L-Selenomethionine2×HCID 7163124-fluoro-L-phenylalanine4×

Product 1

HCID 18649666(2S)-2-(fluoroamino)-3-phenylpropanoic acid

Procedure

Replacement of phenylalanine residues in a protein with p-fluoro-L-phenylalanine (F-Phe) can be implemented for the structural analysis of the protein by19F NMR8, 9. However, as with selenomethionine substitution, F-Phe substitution is cytotoxic. We followed the expression of the CspA in a manner analogous to that demonstrated for selenomethionine EnvZB. CspA was expressed with pColdI(SP4)cspA for 21 hr with and without the addition of F-Phe (FIG. 4A). The addition of F-Phe did not adversely affect CspA production in the SPP system. We then affinity purified the 86-residue CspA product and used mass spectroscopy to determine the extent of F-Phe substitution (FIGS. 4B,C). Although the endogenous Phe biosynthesis was not inhibited in the present SPP system, substantial incorporation of F-Phe (approximately 60 to 70% of total Phe residues) was observed, indicating that toxic F-Phe can be efficiently incorporated into a protein without affecting its yield.

Workup

  1. customWe then affinity purified the 86-residue CspA product
  2. customyield

Sources & evidence

ORD record
ord-3bb84a59155b49839580306f25c26e38
Source dataset
uspto-grants-2011_07
Patent number
US07985575B2

Reaction notes

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