HCID 3283879, compound → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

Participants

Reaction Conditions

Reaction Steps

5-Iodoacetamido fluorescein (5-IAF, 11 mg, 21 μmol) solution in N,N-dimethylformamide (DMF, 50 μL) was slowly added to oxyhemoglobin (60 mg/mL, 5 mL) in 50 mM potassium phosphate pH 7.0 with stirring at 4° C. After three hours of reaction at 4° C., the excess of 5-IAF was removed by extensive dialysis against 50 mM potassium phosphate pH 7.2 until no 5-IAF could be detected in the dialysate. The UV-visible absortion spectrum of the product showed a characteristic fluorescein absorption band at 496 nm.

Provenance & Evidence