HCID 3283879, compound → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

HRID 1017980Notes

Reaction equation

[CH:1]1[C:6](NC(CI)=O)=[CH:5][C:4]([C:12]([OH:14])=[O:13])=[C:3]([C:15]2[C:25]3[CH:26]=[CH:27][C:28]([OH:30])=[CH:29][C:24]=3[O:23][C:22]3[C:16]=2[CH:17]=[CH:18][C:19]([CH:21]=3)=[O:20])[CH:2]=1>CN(C)C=O.P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH:1]1[CH:6]=[CH:5][C:4]([C:12]([OH:14])=[O:13])=[C:3]([C:15]2[C:16]3[CH:17]=[CH:18][C:19]([OH:20])=[CH:21][C:22]=3[O:23][C:24]3[C:25]=2[CH:26]=[CH:27][C:28]([CH:29]=3)=[O:30])[CH:2]=1

Participants

Conditions

Temperature: 4 °C

Procedure

5-Iodoacetamido fluorescein (5-IAF, 11 mg, 21 μmol) solution in N,N-dimethylformamide (DMF, 50 μL) was slowly added to oxyhemoglobin (60 mg/mL, 5 mL) in 50 mM potassium phosphate pH 7.0 with stirring at 4° C. After three hours of reaction at 4° C., the excess of 5-IAF was removed by extensive dialysis against 50 mM potassium phosphate pH 7.2 until no 5-IAF could be detected in the dialysate. The UV-visible absortion spectrum of the product showed a characteristic fluorescein absorption band at 496 nm.

Workup

customAfter three hours of reaction at 4° C.
customthe excess of 5-IAF was removed by extensive dialysis against 50 mM potassium phosphate pH 7.2 until no 5-IAF
customa characteristic fluorescein absorption band at 496 nm

Sources & evidence

ORD record: ord-74068884062943babb1cbb1fc6f36c98
Source dataset: uspto-grants-2002_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US06479637B1

Reaction notes

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