N-bromobutanimide → HCID 69651329, compound

HRID 1018037Notes

Reaction equation

HRID 1018037 reaction equation
C1C(=O)N(Br)C(=O)C1.[CH:9]1[C:13]2=[C:14]([NH2:18])[N:15]=[CH:16][N:17]=[C:12]2[N:11]([C@@H:19]2[O:23][C@H:22]([CH2:24][OH:25])[C@@H:21]([OH:26])[CH2:20]2)[CH:10]=1.C[C:28]([O-:30])=[O:29].[Na+]>CN(C=O)C>[NH2:18][C:14]1[C:13]2[C:9]([C:28]([OH:30])=[O:29])=[CH:10][N:11]([C@@H:19]3[O:23][C@H:22]([CH2:24][OH:25])[C@@H:21]([OH:26])[CH2:20]3)[C:12]=2[N:17]=[CH:16][N:15]=1

Participants

5

Reactant 3

HCID 67184N-bromobutanimideHCID 517045Sodium AcetateHCID 300622260129-59-1

Product 1

HCID 69651329HCID 69651329, compound

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent

Procedure

NBS (1.42 g, 8.0 mmol, dissolved in 4 ml of dried DMF) is added, at room temperature, to a solution of 2′-deoxytubercidin (1.0 g, 4.0 mmol) and NaOAc (0.78 g, 8.0 mmol) in dried DMF (10 ml). The red solution is stirred for 10 minutes and then evaporated. Title compound (38) is obtained as colorless crystals (400 mg, 25%) following flash chromatography (column: 20×5 cm, CH2Cl2/MeOH, 9:1), evaporation of the more rapidly migrating zone and subsequent recrystallization from isopropanol. Isolation of the more slowly migrating zone yields 5-bromo-2′-deoxytubercidin (130 mg, 10%). Method B: a solution of NBS (1.42 g, 8.0 mmol, dissolved in 4 ml of dried DMF) is added to a solution of 5-bromo-2′-deoxytubercidin (1.3 g, 4.0 mmol) and NaOAc (0.785 g, 8.0 mmol) in dried DMF (10 ml). Purification, see method A. Yield: 490 mg, 30% of 21. Melting point: 181° C. TLC (silica gel, CH2Cl2/MeOH, 9:1): Rf 0.40. 1H-NMR (D6-DMSO): δ=2.12 (m, Hα-2′); 2.50 (m, Hβ-2′); 3.51 (m, 2H, H-5′); 3.84 (m, 1H, H-4′); 4.44 (m, 1H, H-3′); 5.20, (br, 2H, 5′-OH, 3′-OH); 6.42 (“t”, J=6.2 Hz, 1H, H-1′); 6.93 (br, 2H, NH2); 8.10 (s, 1H, H-2).

Workup

  1. customevaporated

Sources & evidence

ORD record
ord-b62b9abe296d4d938791c71a9a50282a
Source dataset
uspto-grants-2002_11
Patent number
US06479651B1

Reaction notes

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