反应详情

温度

90 °C

To a 100 mL round-bottomed flask equipped with a reflux condenser was added 2-(4-fluorophenyl)-5-(2-methoxy-5-(1-(pyridin-2-yl)cyclopropylcarbamoyl)phenyl)-N-methylbenzofuran-3-carboxamide (400 mg, 0.747 mmol), DCE (42 mL) and boron tribromide-methyl sulfide complex (1868 mg, 5.97 mmol). The solution was placed under nitrogen and stirred overnight at 90° C. The reaction mixture was cooled to room temperature, quenched with methanol and evaporated to near dryness. The mixture was taken up in 10 mL of methanol and purified using a Shimadzu preparative HPLC employing methanol/water/0.1% trifluoroacetic acid where solvent A was 10% methanol/90% water/0.1% trifluoroacetic acid and solvent B was 10% water/90% methanol/0.1% trifluoroacetic acid with a Phenomenex-Luna 10 μm C18 30×100 mm column at a gradient of 40-100% B and a flow rate of 40 mL/min. over 10 minutes with a 5 minute hold. The solvent was evaporated giving 234 mgs (60% yield) of 2-(4-fluorophenyl)-5-(2-hydroxy-5-(1-(pyridin-2-yl)cyclopropylcarbamoyl)phenyl)-N-methylbenzofuran-3-carboxamide as a white solid. The LC/MS data was obtained on a Shimadzu analytical LC/Micromass Platform LC (ESI+) at 220 nm using the following set of conditions: Sunfire 5 μm C18, 4.6×50 mm column, with a gradient of 0-100% B (B=90% HPLC grade methanol/0.1% trifluoroacetic acid/10% HPLC grade water), (A=90% HPLC grade water/0.1% trifluoroacetic acid/10% HPLC grade methanol), in 4 minutes with a 1 minute hold at a rate of 4 mL/minute. 1H NMR (400 MHz, THF-d8) δ ppm 1.25-1.31 (m, 2 H), 1.59-1.66 (m, 2 H), 2.90 (d, J=4.52 Hz, 3 H), 6.91 (d, J=8.53 Hz, 1 H), 7.08 (dd, J=6.90, 5.40 Hz, 1 H), 7.17-7.24 (m, 2 H), 7.45-7.65 (m, 5 H), 7.79 (dd, J=8.41, 2.13 Hz, 1 H), 7.88 (d, J=1.25 Hz, 1 H), 7.93 (d, J=2.01 Hz, 1 H), 8.08-8.17 (m, 2 H), 8.39-8.45 (m, 1 H), 8.49 (br. s., 2 H). LCMS Rt=1.495 min., m/z 522.33 (M+H), 94% purity.

1. customTo a 100 mL round-bottomed flask equipped with a reflux condenser
2. temperatureThe reaction mixture was cooled to room temperature
3. customquenched with methanol
4. customevaporated
5. custompurified
6. waita flow rate of 40 mL/min. over 10 minutes with a 5 minute hold
7. customThe solvent was evaporated