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HRID1031063

反应详情

EQUATION

反应方程式

HRID 1031063 的结构方程式

REACTANTS

反应物

2
反应SMILES：[O-]B([O-])[O-]
HCID26574

Borate

BO3-3

反应SMILES：O=C(CCCCC1SCC2NC(=O)NC21)NCCCCCC(=O)ON1C(=O)CCC1=O
HCID5063989

Biotin-X-NHS

C20H30N4O6S

PRODUCTS

产物

1
反应SMILES：O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
HCID171548

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
反应SMILES：[Cl-].[Na+]
溶剂HCID5234

Sodium chloride

ClNa

PROCEDURE

实验过程

50 mg bovine serum albumin (BSA) (Fraction V, Fluka) were dissolved in pH 8.5 50 mM NaCl, 20 mM borate buffer. A solution of biotin X NHS (20 mg/200 μl anhydrous dimethylformamide) was then added, and the following steps were identical to those described for the preparation of biotinylated XO.

WORKUP

后处理

  1. customthose described for the preparation of biotinylated XO